Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Talanta. 2010 Mar 15;80(5):1692-7. doi: 10.1016/j.talanta.2009.10.008. Epub 2009 Oct 13.
An effective method for immobilization of Ru(bpy)(3)(2+) on glassy carbon electrode surface (GCE) is developed for the preparation of a novel electrochemiluminescence sensor. First of all, the positively charged Ru(bpy)(3)(2+) is modified on the surface of negatively charged gold nanoparticles (nano-Au) via the electrostatic interactions to obtain the Ru(bpy)(3)(2+)/nano-Au nano-sphere (abbreviate as Ru-AuNPs). Subsequently, the large amount of Ru-AuNPs are immobilized on the multi-wall carbon nanotubes (MWCNTs)-Nafion homogeneous composite coated GCE by dual interaction: firstly, the Nafion, a kind of typical cation-exchange membrane, can absorb the Ru-AuNPs as the enrichment of cation Ru(bpy)(3)(2+) on the Ru-AuNPs surface; secondly, the employment of carboxylic MWCNTs in the Nafion film can also chemosorb the Ru(bpy)(3)(2+) cation on the Ru-AuNPs surface to increase the carrier content. At the same time, the experiment confirms that the enhancement of the ECL intensity on the sensor is attributed to following reasons. One hand, the employment of MWCNTs in the Nafion film enlarged the electro-active surface areas to benefit the contact between the signal probe on the composite film and coreactant used as reinforcing agent. On the other hand, the nano-materials of MWCNTs and nano-Au also improve the conductivity of the assembled film to increase the quantity of excited state of Ru(bpy)(3)(2+) in the unit time under the electrochemical condition and finally cause better properties in luminescence. In the experiment, the influence of the coreactant tripropylamine (TPA) on proposed ECL sensor is investigated. The logarithm of ECL intensity is proportional to the logarithm of TPA concentration on the range of 4 x 10(-10)M to 2.8 x 10(-6)M and 2.8 x 10(-6)M to 0.71 x 10(-3)M. After optimizing these conditions, the ECL sensor with TPA as coreactant is employed to detect a kind of alkaloid medicine, Matrine, for evaluating the practical application in the medicine analysis. The present sensor with TPA as coreactant shows the good response to the medicine concentration of the Matrine from 2.0 x 10(-6)M to 6.0 x 10(-3)M, which is used to detect the Matrine concentration in the Matrine injection.
一种将 Ru(bpy)(3)(2+)固定在玻碳电极表面（GCE）上的有效方法被开发出来，用于制备新型的电化学发光传感器。首先，通过静电相互作用将带正电荷的 Ru(bpy)(3)(2+)修饰在带负电荷的金纳米粒子（nano-Au）表面上，得到 Ru(bpy)(3)(2+)/nano-Au 纳米球（简称 Ru-AuNPs）。随后，大量的 Ru-AuNPs 通过双重相互作用固定在多壁碳纳米管（MWCNTs）-Nafion 均匀复合涂层 GCE 上：首先，Nafion 作为一种典型的阳离子交换膜，可以吸收 Ru-AuNPs，作为 Ru-AuNPs 表面上 Ru(bpy)(3)(2+)阳离子的富集；其次，在 Nafion 膜中使用羧酸化 MWCNTs 也可以化学吸附 Ru-AuNPs 表面上的 Ru(bpy)(3)(2+)阳离子，以增加载体含量。同时，实验证实传感器上的 ECL 强度增强归因于以下原因。一方面，MWCNTs 在 Nafion 膜中的使用扩大了电活性表面积，有利于复合膜上信号探针与增强剂核心反应物之间的接触。另一方面，MWCNTs 和纳米金等纳米材料也提高了组装膜的导电性，在电化学条件下，单位时间内增加 Ru(bpy)(3)(2+)激发态的数量，最终在发光性能方面表现更好。在实验中，研究了增强剂三丙胺（TPA）对所提出的 ECL 传感器的影响。在 4×10^(-10)M 至 2.8×10^(-6)M 和 2.8×10^(-6)M 至 0.71×10^(-3)M 的范围内，ECL 强度的对数与 TPA 浓度的对数成正比。在优化这些条件后，使用 TPA 作为核心反应物的 ECL 传感器用于检测一种生物碱药物苦参碱，以评估其在药物分析中的实际应用。以 TPA 为核心反应物的本传感器对苦参碱的药物浓度从 2.0×10^(-6)M 至 6.0×10^(-3)M 表现出良好的响应，用于检测苦参碱注射液中的苦参碱浓度。
Biosensors (Basel). 2022-12-30
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