Department of Physical and Analytical Chemistry, Uppsala University, Box 599, SE-751 24 Uppsala, Sweden.
Talanta. 2010 Mar 15;80(5):2068-75. doi: 10.1016/j.talanta.2009.11.007. Epub 2009 Nov 12.
A fast method for direct multi-element analysis of non-digested biological samples is presented. The only sample preparation needed is 1 min homogenization with a Polytron mixer in a small volume of neutral phosphate buffer saline solution (PBS). The total time for analysis (sample preparation and measurement) is 4 min only. This "mix and measure" method can handle large sample loads of biological samples and thus minimize dilution of trace elements. For example 100% whole blood was introduced without any clogging of the introduction system or extinguishing of the plasma. In 70% (v/v) whole blood reference material 14 of 16 analytes were quantified within +/-10% (Al, B, Ba, Ca, Cu, Fe, Mg, Mn, P, Pb, S, Sr, Ti and Zn) and two semi-quantified within +/-20% (Cd and K). Fresh bovine liver was also analyzed with the same method and 7 of 9 analytes were quantified in 5% (w/v) liver slurry. Three different nebulizers were tested, Glass Expansion Concentric (GEC) of Meinhard type, Cross Flow and Burgener T2100 and they performed roughly equally well in giving quantitative results for the slurries but the sensitivity was better with the GEC. The stability of the plasma was studied by evaluating the ratio of Mg 280.270 nm and Mg 285.213 nm lines. When increasing the sample load from 20 to 100% (v/v) of whole blood and from 0.5 to 10% (w/v) of bovine liver the Mg ratio was constant within a few percent for all of the nebulizer tested. The ratio of the sensitivity between GEC and Burgener T2100 was studied and the ratio increased with the energy sum for atomic and ionic lines separately.
本文提出了一种快速直接测定未经消解的生物样品中多种元素的方法。唯一需要的样品制备是在中性磷酸盐缓冲盐水（PBS）溶液中用 Polytron 混合器进行 1 分钟的匀浆。分析的总时间（样品制备和测量）仅为 4 分钟。这种“混合和测量”方法可以处理大量生物样品，从而最大限度地减少微量元素的稀释。例如，100%全血可以直接引入，而不会堵塞进样系统或熄灭等离子体。在 70%（v/v）全血参考物质中，16 种分析物中的 14 种被定量在 +/-10%（Al、B、Ba、Ca、Cu、Fe、Mg、Mn、P、Pb、S、Sr、Ti 和 Zn）范围内，两种被半定量在 +/-20%（Cd 和 K）范围内。同样的方法也用于分析新鲜牛肝，在 5%（w/v）肝浆中定量了 7 种分析物。测试了三种不同的雾化器，Meinhard 型玻璃扩张同心（GEC）、交叉流和 Burgener T2100，它们在提供浆状物定量结果方面表现大致相同，但 GEC 的灵敏度更好。通过评估 Mg 280.270nm 和 Mg 285.213nm 谱线的比值来研究等离子体的稳定性。当将全血样品的负载从 20%（v/v）增加到 100%（v/v），以及将牛肝样品的负载从 0.5%（w/v）增加到 10%（w/v）时，所有测试的雾化器中 Mg 比值在几个百分点内保持不变。研究了 GEC 和 Burgener T2100 之间的灵敏度比，并且该比值随着原子和离子谱线的能量总和的增加而增加。
