Department of Biochemistry, Dokkyo University School of Medicine, Tochigi, Japan.
Plant Physiol Biochem. 2010 Jul;48(7):591-5. doi: 10.1016/j.plaphy.2010.01.018. Epub 2010 Jan 28.
The synthesis and translocation of the diamine cadaverine during soybean (Glycine max L. Meer cv. Sakai) germination were studied using 15N-labelled lysine (the cadaverine precursor) and 15N-labelled cadaverine, both under light/dark (12 h/12 h) and total dark germinating conditions. 15N-cadaverine and non-labelled polyamines were simultaneously detected using ionspray ionization-mass spectrometry. Both 15N-cadaverine and 15N-lysine were taken up by soybean. 15N-lysine was transported to the shoot and root and converted into 15N-cadaverine, whereas relatively little 15N-cadaverine was formed from 15N-lysine in the cotyledon. The acropetal translocation of 15N-cadaverine from the cotyledon to the shoot seemed to predominate over basipetal transport to the root. Although no other 15N-derivatised polyamines were found, supplying exogenous 15N-lysine seemed to indirectly affect the metabolism of 14N putrescine, spermidine and spermine, while no significant effect was detected after supplying 15N-cadaverine.
在光照/黑暗（12 小时/12 小时）和完全黑暗发芽条件下，使用 15N 标记的赖氨酸（腐胺前体）和 15N 标记的腐胺研究了大豆（Glycine max L. Meer cv. Sakai）发芽过程中二元胺腐胺的合成和转运。使用离子喷雾电离-质谱法同时检测 15N-腐胺和非标记多胺。大豆吸收了 15N-腐胺和 15N-赖氨酸。15N-赖氨酸被转运到地上部和根部，并转化为 15N-腐胺，而在子叶中，15N-赖氨酸转化为 15N-腐胺的量相对较少。15N-腐胺从子叶向地上部的向顶转运似乎优先于向根的向基运输。尽管没有发现其他 15N 衍生的多胺，但外源 15N-赖氨酸的供应似乎会间接影响 14N 腐胺、亚精胺和精胺的代谢，而供应 15N-腐胺后则没有检测到明显的影响。
