State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of China.
Hum Immunol. 2010 Jun;71(6):586-91. doi: 10.1016/j.humimm.2010.02.010. Epub 2010 Mar 7.
Immune thrombocytopenia (ITP) is an acquired organ-specific autoimmune disease with a polarization of T(h)1. Both the T(h)1 chemokine CXCL10 and T(h)2 chemokine CCL2 have been studied in several autoimmune diseases, but the status of these chemokines in ITP is still unknown. The aims of this study were to determine the expression of CXCL10 and CCL2 and their receptors, CXCR3 and CCR2, in ITP patients, and to conduct a preliminary study of the pathogenic roles of these factors in ITP. Plasma samples from 49 patients with ITP and 24 normal healthy subjects were assayed for CXCL10 and CCL2 plasma concentration by enzyme-linked immunosorbent assay. Real-time quantitative polymerase chain reaction was performed to determine the mRNA expression of these chemokines and their receptors in the PBMNC of 24 normal controls and 28 active ITP patients as well as splenocytes of nine ITP patients. The CXCL10 levels in the plasma samples from patients with active ITP were significantly higher than those from healthy controls (p = 0.007) and decreased to normal levels in patients with remission ITP. In contrast, CCL2 levels were similar in patients with active disease, patients in remission, and control subjects. PBMNC of patients with active disease expressed more CXCL10 mRNA (p = 0.031) but less CCR2 mRNA (p = 0.005). Lower peripheral platelet count correlated with higher CXCL10 levels and CXCL10/CCL2 ratios. Our study demonstrated that plasma levels of CXCL10 and CXC10/CCL2 ratio were higher in patients with active ITP than in healthy donors, and had an association with platelet counts of the patients. CXCL10 might be a pathogenic factor of this disorder.
免疫性血小板减少症 (ITP) 是一种获得性器官特异性自身免疫性疾病，其 T(h)1 极化。几种自身免疫性疾病中均研究了 T(h)1 趋化因子 CXCL10 和 T(h)2 趋化因子 CCL2，但它们在 ITP 中的状态仍不清楚。本研究旨在确定 ITP 患者中 CXCL10 和 CCL2 及其受体 CXCR3 和 CCR2 的表达，并对这些因子在 ITP 中的致病作用进行初步研究。通过酶联免疫吸附试验测定 49 例 ITP 患者和 24 例正常健康受试者的血浆样本中 CXCL10 和 CCL2 的血浆浓度。通过实时定量聚合酶链反应测定 24 例正常对照者和 28 例活动性 ITP 患者的 PBMNC 以及 9 例 ITP 患者的脾细胞中这些趋化因子及其受体的 mRNA 表达。结果显示，活动性 ITP 患者的血浆样本中 CXCL10 水平明显高于健康对照组（p = 0.007），且在缓解性 ITP 患者中降至正常水平。相比之下，活动性疾病患者、缓解性患者和对照组患者的 CCL2 水平相似。活动性疾病患者的 PBMNC 表达更多的 CXCL10 mRNA（p = 0.031），但表达更少的 CCR2 mRNA（p = 0.005）。外周血小板计数越低，与 CXCL10 水平和 CXCL10/CCL2 比值越高呈正相关。本研究表明，活动性 ITP 患者的血浆 CXCL10 水平和 CXCL10/CCL2 比值高于健康供体，且与患者的血小板计数有关。CXCL10 可能是该疾病的一个致病因素。
