采用电喷雾和大气压光离子化傅里叶变换离子回旋共振质谱对海洋溶解有机物进行全面表征。

Comprehensive characterization of marine dissolved organic matter by Fourier transform ion cyclotron resonance mass spectrometry with electrospray and atmospheric pressure photoionization.

机构信息

Department of Chemistry & Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USA.

出版信息

Rapid Commun Mass Spectrom. 2010 Mar 15;24(5):643-50. doi: 10.1002/rcm.4421.

PMID:20155760

Abstract

We compare the ultrahigh resolution 9.4 T Fourier transform ion cyclotron resonance (FT-ICR) mass spectra of marine dissolved organic matter (DOM) isolated from two sites in the Weddell Sea (Antarctica) obtained by complementary electrospray ionization (ESI) and atmospheric pressure photoionization (APPI). Ions produced by APPI extend to higher carbon unsaturation than those produced by ESI, indicated by higher double-bond equivalents (rings plus double bonds) minus oxygen (DBE-O) values, whereas ESI-generated ions are more oxygenated. Moreover, many sulfur-containing compounds were efficiently ionized by ESI but not detected by APPI. Because the mass spectra obtained by ESI and APPI are significantly different, both are necessary to obtain a more complete description of the molecular composition of marine DOM.

摘要

我们比较了通过互补电喷雾电离（ESI）和大气压光致电离（APPI）从南极威德尔海（Antarctica）两个站点分离得到的海洋溶解有机物（DOM）的超高分辨率 9.4 T 傅里叶变换离子回旋共振（FT-ICR）质谱。APPI 产生的离子比 ESI 产生的离子具有更高的碳不饱和性，这表现为更高的双键等价物（环加双键）减去氧（DBE-O）值，而 ESI 产生的离子则具有更高的含氧性。此外，许多含硫化合物可以通过 ESI 有效地离子化，但不能通过 APPI 检测到。由于 ESI 和 APPI 获得的质谱有很大的不同，因此两者都是获得更完整的海洋 DOM 分子组成描述所必需的。