Department of Molecular Biology, Kyushu University Graduate School of Pharmaceutical Sciences, Fukuoka 812-8582, Japan.
Nat Rev Microbiol. 2010 Mar;8(3):163-70. doi: 10.1038/nrmicro2314.
Chromosomal replication must be limited to once and only once per cell cycle. This is accomplished by multiple regulatory pathways that govern initiator proteins and replication origins. A principal feature of DNA replication is the coupling of the replication reaction to negative-feedback regulation. Some of the factors that are important in this process have been discovered, including the clamp (DNA polymerase III subunit-beta (DnaN)), the datA locus, SeqA, DnaA homologue protein (Hda) and YabA, as well as factors that are involved at other stages of the regulatory mechanism, such as DnaA initiator-associating protein (DiaA), the DnaA-reactivating sequence (DARS) loci and Soj. Here, we describe the regulation of DnaA, one of the central proteins involved in bacterial DNA replication, by these factors in Escherichia coli, Bacillus subtilis and Caulobacter crescentus.
染色体复制必须限制在每个细胞周期中仅进行一次。这是通过多个调节途径来实现的，这些途径控制着起始蛋白和复制原点。DNA 复制的一个主要特征是将复制反应与负反馈调节偶联。在这个过程中发现了一些重要的因素，包括夹子（DNA 聚合酶 III 亚基-β（DnaN））、datA 基因座、SeqA、DnaA 同源蛋白（Hda）和 YabA，以及在调节机制的其他阶段涉及的因素，如 DnaA 起始蛋白结合蛋白（DiaA）、DnaA 再激活序列（DARS）基因座和 Soj。在这里，我们描述了大肠杆菌、枯草芽孢杆菌和新月柄杆菌中这些因素对细菌 DNA 复制中核心蛋白之一 DnaA 的调节。
