[建立糖尿病性勃起功能障碍大鼠阴茎海绵体平滑肌细胞的细胞培养模型]
[Establishment of a cell culture model of corpus cavernosum smooth muscle cells derived from diabetic rats with erectile dysfunction].
作者信息
Ye Ting-yu, Wei An-yang, Wan Bo, Yang Yong, Luo Xin-gui
机构信息
Huiqiao Department, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Feb;30(2):351-4.
OBJECTIVE
To investigate the method for culturing corpus cavernosum smooth muscle cells (CCSMs) derived from diabetic rats with erectile dysfunction (ED) for the study of ED caused by diabetes.
METHODS
CCSMs were isolated from the corpus cavernosum of diabetic rats with ED and cultured using a modified method of adherent tissue culture. The cultured cells were identified by immunohistochemistry and the cell morphology and proliferation were observed.
RESULTS
The primary culture of CCSM was performed successfully, and the cells were seen to migrate from the small tissue pieces 3 days later, reaching nearly confluence in 16-18 days. A typical "hill-valley" growth pattern was noted in the cell passaging. Immunohistochemical staining for alpha-smooth muscle actin (alpha-SM-actin) and desmin yielded positive results in the cells.
CONCLUSION
The modified method for adherent tissue culture is convenient and reliable in establishing the in vitro cell culture model of CCSMs from diabetic rats with ED, and the cultured CCSMs display a faster proliferation than normal CCSMs. No obvious differences in the cell morphology can be found between diabetic and normal CCSMs under light microscope.
目的
研究培养来自患有勃起功能障碍（ED）的糖尿病大鼠的海绵体平滑肌细胞（CCSMs）的方法，用于糖尿病所致ED的研究。
方法
从患有ED的糖尿病大鼠的海绵体中分离出CCSMs，采用改良的贴壁组织培养方法进行培养。通过免疫组织化学鉴定培养的细胞，并观察细胞形态和增殖情况。
结果
成功进行了CCSM的原代培养，3天后可见细胞从小组织块中迁移出来，16 - 18天达到近汇合状态。细胞传代时呈现典型的“峰谷”生长模式。对α-平滑肌肌动蛋白（α-SM-肌动蛋白）和结蛋白的免疫组织化学染色在细胞中呈阳性结果。
结论
改良的贴壁组织培养方法在建立来自患有ED的糖尿病大鼠的CCSMs体外细胞培养模型方面方便可靠，且培养的CCSMs比正常CCSMs增殖更快。在光学显微镜下，糖尿病CCSMs和正常CCSMs之间在细胞形态上未发现明显差异。
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