Jonkisz Paweł, Kungl Krisztina, Sikorska Agnieszka, Kurosad Agnieszka, Nicpoń Józef
Wrocław University of Environmental and Life Sciences Department of Internal and Parasitic Diseases with Clinic for Horses, Dogs And Cats, Faculty of Veterinary Medicine Pl. Grunwaldzki 47 50-366 Wrocław Poland.
Acta Vet Hung. 2010 Mar;58(1):59-67. doi: 10.1556/AVet.58.2010.1.6.
Cystatin C is a serum protein with low molecular mass, which has been suggested as a marker to assess renal function in the dog. This protein is regularly assessed using particle-enhanced turbidimetric immunoassay (PETIA) and particle-enhanced nephelometric immunoassay (PENIA), in which rabbit anti-human cystatin C antibodies are used. The purpose of this work was to compare the results of cystatin C analysis obtained by PETIA and PENIA assays in the dog. Forty dogs of different genders and breeds were classified into four groups of 10 animals each based on serum creatinine concentrations (4 stages of chronic kidney disease). Serum cystatin C concentration was measured using PETIA and PENIA assays, the results were compared, and correlation with serum urea and creatinine concentrations was established. The correlation coefficient for results obtained using PETIA and PENIA assays was r = 0.706. Serum cystatin C concentrations obtained in PETIA had a lower correlation coefficient with creatinine concentrations than those found in PENIA (r = 0.614 and r = 0.904, respectively); similarly, serum cystatin C was less correlated with serum urea concentration in PETIA than in PENIA (r = 0.463 and r = 0.636, respectively). The results obtained in this study suggest that the nephelometric assay is more sensitive and was shown to be more closely correlated with other renal function indicators than the PETIA assay.
胱抑素C是一种低分子量的血清蛋白，已被提议作为评估犬肾功能的标志物。该蛋白通常使用颗粒增强比浊免疫测定法（PETIA）和颗粒增强散射免疫比浊法（PENIA）进行评估，这两种方法均使用兔抗人胱抑素C抗体。本研究的目的是比较PETIA和PENIA检测法在犬中获得的胱抑素C分析结果。根据血清肌酐浓度（慢性肾病的4个阶段），将40只不同性别和品种的犬分为4组，每组10只动物。使用PETIA和PENIA检测法测量血清胱抑素C浓度，比较结果，并建立与血清尿素和肌酐浓度的相关性。PETIA和PENIA检测法所得结果的相关系数为r = 0.706。PETIA法测得的血清胱抑素C浓度与肌酐浓度的相关系数低于PENIA法（分别为r = 0.614和r = 0.904）；同样，PETIA法中血清胱抑素C与血清尿素浓度的相关性低于PENIA法（分别为r = 0.463和r = 0.636）。本研究获得的结果表明，散射免疫比浊法更敏感，并且与其他肾功能指标的相关性比PETIA法更强。
