Suppr超能文献

MccE 通过乙酰化抗生素的加工形式提供对蛋白质合成抑制剂微菌素 C 的抗性。

MccE provides resistance to protein synthesis inhibitor microcin C by acetylating the processed form of the antibiotic.

机构信息

Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.

出版信息

J Biol Chem. 2010 Apr 23;285(17):12662-9. doi: 10.1074/jbc.M109.080192. Epub 2010 Feb 16.

Abstract

The heptapeptide-nucleotide microcin C (McC) is a potent inhibitor of enteric bacteria growth. McC is excreted from producing cells by the MccC transporter. The residual McC that remains in the producing cell can be processed by cellular aminopeptidases with the release of a non-hydrolyzable aspartyl-adenylate, a strong inhibitor of aspartyl-tRNA synthetase. Accumulation of processed McC inside producing cells should therefore lead to translation inhibition and cessation of growth. Here, we show that a product of another gene of the McC biosynthetic cluster, mccE, acetylates processed McC and converts it into a non-toxic compound. MccE also makes Escherichia coli resistant to albomycin, a Trojan horse inhibitor unrelated to McC that, upon processing, gives rise to a serine coupled to a thioxylofuranosyl pyrimidine, an inhibitor of seryl-tRNA synthetase. We speculate that MccE and related cellular acetyltransferases of the Rim family may detoxify various aminoacyl-nucleotides, either exogenous or those generated inside the cell.

摘要

七肽核苷酸类微菌素 C(McC)是一种强效的肠道细菌生长抑制剂。McC 通过 MccC 转运蛋白从产生细胞中分泌出来。残留在产生细胞中的残留 McC 可以被细胞氨肽酶处理,释放出不可水解的天冬氨酰-腺苷酸,这是天冬氨酰-tRNA 合成酶的强抑制剂。因此,产生细胞内积累的加工 McC 应该会导致翻译抑制和生长停止。在这里,我们表明 McC 生物合成簇的另一个基因 mccE 的产物乙酰化加工后的 McC,并将其转化为无毒化合物。MccE 还使大肠杆菌对 albomycin 产生抗性,albomycin 是一种与 McC 无关的特洛伊木马抑制剂,在加工后会产生与硫氧呋喃嘧啶连接的丝氨酸,这是丝氨酰-tRNA 合成酶的抑制剂。我们推测 MccE 和 Rim 家族的相关细胞乙酰转移酶可能会解毒各种氨酰核苷酸,无论是外源性的还是细胞内产生的。

相似文献

1
MccE provides resistance to protein synthesis inhibitor microcin C by acetylating the processed form of the antibiotic.
J Biol Chem. 2010 Apr 23;285(17):12662-9. doi: 10.1074/jbc.M109.080192. Epub 2010 Feb 16.
2
The RimL transacetylase provides resistance to translation inhibitor microcin C.
J Bacteriol. 2014 Oct;196(19):3377-85. doi: 10.1128/JB.01584-14. Epub 2014 Jul 7.
3
The mechanism of microcin C resistance provided by the MccF peptidase.
J Biol Chem. 2010 Dec 3;285(49):37944-52. doi: 10.1074/jbc.M110.179135. Epub 2010 Sep 27.
4
Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
J Bacteriol. 2008 Apr;190(7):2607-10. doi: 10.1128/JB.01956-07. Epub 2008 Jan 25.
5
Structural basis for microcin C7 inactivation by the MccE acetyltransferase.
J Biol Chem. 2011 Jun 17;286(24):21295-303. doi: 10.1074/jbc.M111.226282. Epub 2011 Apr 19.
6
Maturation of the translation inhibitor microcin C.
J Bacteriol. 2009 Apr;191(7):2380-7. doi: 10.1128/JB.00999-08. Epub 2009 Jan 23.
7
Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic Microcin C.
J Biol Chem. 2006 Jun 30;281(26):18033-42. doi: 10.1074/jbc.M513174200. Epub 2006 Mar 30.
8
Structural and functional characterization of microcin C resistance peptidase MccF from Bacillus anthracis.
J Mol Biol. 2012 Jul 20;420(4-5):366-83. doi: 10.1016/j.jmb.2012.04.011. Epub 2012 Apr 16.
9
Amino acid residues required for maturation, cell uptake, and processing of translation inhibitor microcin C.
J Bacteriol. 2007 Mar;189(5):2114-8. doi: 10.1128/JB.01609-06. Epub 2006 Dec 8.
10
The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C.
J Bacteriol. 2007 Nov;189(22):8361-5. doi: 10.1128/JB.01028-07. Epub 2007 Sep 14.

引用本文的文献

2
Decarboxylation in Natural Products Biosynthesis.
JACS Au. 2024 Jul 25;4(8):2715-2745. doi: 10.1021/jacsau.4c00425. eCollection 2024 Aug 26.
4
Exploring the genetic basis of natural resistance to microcins.
Microb Genom. 2024 Feb;10(2). doi: 10.1099/mgen.0.001156.
5
Natural Trojan horse inhibitors of aminoacyl-tRNA synthetases.
RSC Chem Biol. 2021 Feb 22;2(2):468-485. doi: 10.1039/d0cb00208a. eCollection 2021 Apr 1.
6
Gcn5-Related Acetyltransferases (GNATs) With a Catalytic Serine Residue Can Play Ping-Pong Too.
Front Mol Biosci. 2021 Apr 12;8:646046. doi: 10.3389/fmolb.2021.646046. eCollection 2021.
7
Aminoacyl-tRNA Synthetases as Valuable Targets for Antimicrobial Drug Discovery.
Int J Mol Sci. 2021 Feb 10;22(4):1750. doi: 10.3390/ijms22041750.
8
Bacteriocins to Thwart Bacterial Resistance in Gram Negative Bacteria.
Front Microbiol. 2020 Nov 9;11:586433. doi: 10.3389/fmicb.2020.586433. eCollection 2020.
9
Small-Molecule Acetylation by GCN5-Related -Acetyltransferases in Bacteria.
Microbiol Mol Biol Rev. 2020 Apr 15;84(2). doi: 10.1128/MMBR.00090-19. Print 2020 May 20.
10
Histidine-Triad Hydrolases Provide Resistance to Peptide-Nucleotide Antibiotics.
mBio. 2020 Apr 7;11(2):e00497-20. doi: 10.1128/mBio.00497-20.

本文引用的文献

1
Characterization of two seryl-tRNA synthetases in albomycin-producing Streptomyces sp. strain ATCC 700974.
Antimicrob Agents Chemother. 2009 Nov;53(11):4619-27. doi: 10.1128/AAC.00782-09. Epub 2009 Aug 31.
2
How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.
EMBO J. 2009 Jul 8;28(13):1953-64. doi: 10.1038/emboj.2009.146. Epub 2009 Jun 4.
3
Maturation of the translation inhibitor microcin C.
J Bacteriol. 2009 Apr;191(7):2380-7. doi: 10.1128/JB.00999-08. Epub 2009 Jan 23.
4
Maturation of an Escherichia coli ribosomal peptide antibiotic by ATP-consuming N-P bond formation in microcin C7.
J Am Chem Soc. 2008 Mar 19;130(11):3603-9. doi: 10.1021/ja7101949. Epub 2008 Feb 22.
5
Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
J Bacteriol. 2008 Apr;190(7):2607-10. doi: 10.1128/JB.01956-07. Epub 2008 Jan 25.
7
Low-molecular-weight post-translationally modified microcins.
Mol Microbiol. 2007 Sep;65(6):1380-94. doi: 10.1111/j.1365-2958.2007.05874.x. Epub 2007 Aug 17.
8
Amino acid residues required for maturation, cell uptake, and processing of translation inhibitor microcin C.
J Bacteriol. 2007 Mar;189(5):2114-8. doi: 10.1128/JB.01609-06. Epub 2006 Dec 8.
9
Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic Microcin C.
J Biol Chem. 2006 Jun 30;281(26):18033-42. doi: 10.1074/jbc.M513174200. Epub 2006 Mar 30.
10
Exoproducts of the Escherichia coli strain H22 inhibiting some enteric pathogens both in vitro and in vivo.
J Appl Microbiol. 2006 Apr;100(4):821-9. doi: 10.1111/j.1365-2672.2006.02834.x.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验