用于体内研究人体脂肪酸和甘油三酯代谢的稳定同位素标记示踪剂。
Stable isotope-labeled tracers for the investigation of fatty acid and triglyceride metabolism in humans in vivo.
作者信息
Magkos Faidon, Mittendorfer Bettina
机构信息
Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO, USA.
出版信息
Clin Lipidol. 2009 Apr 1;4(2):215-230. doi: 10.2217/clp.09.9.
Understanding lipid metabolism and its regulation requires information on the rates at which lipids are produced within the body, absorbed (dietary lipids) into the body, transported within the body, and utilized by various tissues. This article focuses on the use of stable isotope-labeled tracers for the quantitative evaluation of major pathways of fatty acid and triglyceride metabolism in humans in vivo. Adipose tissue lipolysis and free fatty acid appearance in plasma, fatty acid tissue uptake and oxidation, and hepatic very low-density lipoprotein triglyceride secretion are among the metabolic pathways that can be studied by using stable isotope labeled tracers, and will be discussed in detail. The methodology has been in use for many years and is constantly being refined. A variety of tracers and analytical approaches are available and can be used; knowing the advantages, assumptions, and limitations of each is essential for the planning of studies and the interpretation of data, which can provide unique insights into human lipid metabolism.
了解脂质代谢及其调节需要掌握有关脂质在体内产生、吸收（膳食脂质）进入体内、在体内运输以及被各种组织利用的速率的信息。本文重点介绍使用稳定同位素标记示踪剂对人体内脂肪酸和甘油三酯代谢的主要途径进行体内定量评估。脂肪组织脂解和血浆中游离脂肪酸的出现、脂肪酸组织摄取和氧化以及肝脏极低密度脂蛋白甘油三酯分泌是可以通过使用稳定同位素标记示踪剂进行研究的代谢途径，将进行详细讨论。该方法已经使用多年并且在不断完善。有多种示踪剂和分析方法可供使用；了解每种方法的优点、假设和局限性对于研究规划和数据解释至关重要，这可以为人类脂质代谢提供独特的见解。
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