Antelman Josh, Ebenstein Yuval, Dertinger Thomas, Michalet Xavier, Weiss Shimon
Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, CA 90095 USA.
J Phys Chem C Nanomater Interfaces. 2009 Mar 31;113(27):11541-11545. doi: 10.1021/jp811078e.
In this report we evaluate the emission properties of single quantum dots embedded in a thin, thiol containing polymer film. We report the suppression of quantum dot blinking leading to a continuous photon flux from both organic and water soluble quantum dots and demonstrate their application as robust fluorescent point sources for ultrahigh resolution localization. In addition, we apply the polymer coating to cell samples immunostained with antibody conjugated QDs and show that fluorescence intensity from the polymer embedded cells shows no sign of degradation after 67 hours of continuous excitation. The reported thin polymer film coating may prove advantageous for immuno-cyto/histo-chemistry as well as for the fabrication of quantum dot containing devices requiring a reliable and stable photon source (including a single photon source) or stable charge characteristics while maintaining intimate contact between the quantum dot and the surrounding matrix.
在本报告中，我们评估了嵌入含硫醇聚合物薄膜中的单个量子点的发射特性。我们报道了量子点闪烁的抑制，这导致了来自有机和水溶性量子点的连续光子通量，并展示了它们作为超高分辨率定位的稳健荧光点源的应用。此外，我们将聚合物涂层应用于用抗体偶联量子点免疫染色的细胞样本，并表明在连续激发67小时后，嵌入聚合物的细胞的荧光强度没有降解迹象。所报道的薄聚合物薄膜涂层对于免疫细胞/组织化学以及制造需要可靠且稳定的光子源（包括单光子源）或稳定电荷特性同时保持量子点与周围基质紧密接触的含量子点器件可能是有利的。
