Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.
ACS Appl Mater Interfaces. 2009 Apr;1(4):768-74. doi: 10.1021/am800166t.
A design of smart surfaces responsive to biochemical analytes is demonstrated in the example of mixed monolayers of biotin/fluorocarbon. The contact angle of aqueous solutions on such surfaces decreases upon streptavidin binding and can be used in detecting this protein. The specificity of the effect is confirmed by the lack of a contact angle change by streptavidin blocked with biotin and by bovine serum albumin.
设计了一种对生化分析物有响应的智能表面，以生物素/氟碳混合单层为例。在这种表面上，水溶液的接触角在链霉亲和素结合后会降低，可以用于检测这种蛋白质。通过用生物素封闭的链霉亲和素和牛血清白蛋白没有引起接触角变化，证实了这种效应的特异性。
