Bogne Kamga Patrice, Penlap Beng Véronique, Lontsi David, Etoa François-Xavier
Department of Biochemistry, Faculty of Science, P.O. Box 812, University of Yaounde I.
Afr J Tradit Complement Altern Med. 2007 Feb 16;4(3):352-6. doi: 10.4314/ajtcam.v4i3.31229.
The methanolic extract and conessine isolated from the stem bark of Holarrhena floribunda (Hf) were tested for their antibacterial activities on Bacillus: Bacillus cereus, Bacillus subtilis, Bacillus megaterium and Bacillus stearothermophilus using the disc diffusion method. Phytochemical analysis of the crude extract and fractions was also conducted. The inhibition parameters of the crude methanol extract and the total alkaloid fraction were determined using the macrodilution method. The results showed that the crude extract, the total alkaloid fraction and conessine exhibited a significant antibacterial effect against all the strains studied. The antibacterial effect of conessine is almost similar to that of chloramphenicol used as reference. The ratio of the minimal bactericidal concentration (MBC) over the minimal inhibitive concentration (MIC) indicates the bactericidal effect of the plant. These results support common use of stem bark of Hf and conessine isolated from Hf in the treatment of some infectious diseases.
采用纸片扩散法，对从多花止泻木（Hf）茎皮中分离得到的甲醇提取物和康丝碱针对芽孢杆菌属的蜡样芽孢杆菌、枯草芽孢杆菌、巨大芽孢杆菌和嗜热脂肪芽孢杆菌的抗菌活性进行了测试。还对粗提物和各馏分进行了植物化学分析。采用大稀释法测定了粗甲醇提取物和总生物碱馏分的抑制参数。结果表明，粗提物、总生物碱馏分和康丝碱对所有研究菌株均表现出显著的抗菌作用。康丝碱的抗菌作用与用作对照的氯霉素几乎相似。最低杀菌浓度（MBC）与最低抑菌浓度（MIC）之比表明了该植物的杀菌作用。这些结果支持多花止泻木茎皮和从多花止泻木中分离得到的康丝碱在治疗某些传染病中的常用性。
