Research Group of Functional Genomics and Proteomics, KU Leuven, Naamsestraat 59, Leuven, Belgium.
Biol Direct. 2010 Feb 17;5:11. doi: 10.1186/1745-6150-5-11.
The success of invertebrates throughout evolution is an excellent illustration of the efficiency of their defence strategies. Caenorhabditis elegans has proven to be an appropriate model for transcriptome studies of host-pathogen interactions. The aim of this paper is to complement this knowledge by investigating the worm's response to a Staphylococcus aureus infection through a 2-dimensional differential proteomics approach.
Different types of growth media in combination with either E. coli OP50 or Staphylococcus aureus were tested for an effect on the worm's lifespan. LB agar was chosen and C. elegans samples were collected 1 h, 4 h, 8 h and 24 h post S. aureus infection or E. coli incubation. Proteomics analyses resulted in the identification of 130 spots corresponding to a total of 108 differentially expressed proteins.
Exploring four time-points discloses a dynamic insight of the reaction against a gram-positive infection at the level of the whole organism. The remarkable upregulation after 8 h and 24 h of many enzymes involved in the citric acid cycle might illustrate the cost of fighting off an infection. Intriguing is the downregulation of chaperone molecules, which are presumed to serve a protective role. A comparison with a similar experiment in which C. elegans was infected with the gram-negative Aeromonas hydrophila reveals that merely 9% of the identified spots, some of which even exhibiting an opposite regulation, are present in both studies. Hence, our findings emphasise the complexity and pathogen-specificity of the worm's immune response and form a firm basis for future functional research.
This article was reviewed by Itai Yanai, Dieter Wolf and Torben Luebke (nominated by Walter Lutz).
无脊椎动物在进化过程中的成功是其防御策略效率的极好例证。秀丽隐杆线虫已被证明是研究宿主-病原体相互作用转录组的合适模型。本文旨在通过二维差异蛋白质组学方法研究蠕虫对金黄色葡萄球菌感染的反应，来补充这方面的知识。
研究了不同类型的生长培养基与大肠杆菌 OP50 或金黄色葡萄球菌组合对蠕虫寿命的影响。选择 LB 琼脂，在金黄色葡萄球菌感染或大肠杆菌孵育后 1 h、4 h、8 h 和 24 h 收集线虫样本。蛋白质组学分析鉴定出 130 个斑点，对应于总共 108 个差异表达蛋白。
探索四个时间点可以揭示整个生物体对革兰氏阳性感染的反应的动态见解。8 小时和 24 小时后许多参与柠檬酸循环的酶的显著上调可能表明了抵御感染的代价。引人注目的是伴侣分子的下调，这些分子被认为具有保护作用。与秀丽隐杆线虫感染革兰氏阴性嗜水气单胞菌的类似实验进行比较，发现在两项研究中仅存在 9%的鉴定斑点，其中一些甚至表现出相反的调控，这表明我们的研究结果强调了蠕虫免疫反应的复杂性和病原体特异性，并为未来的功能研究奠定了坚实的基础。
本文由 Itai Yanai、Dieter Wolf 和 Torben Luebke（由 Walter Lutz 提名）进行了评审。
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