Brazilian Society of Plastic Surgery, São Paulo, SP, Brazil; Santa Cruz Plastic Surgery Institute, São Paulo, SP, Brazil.
J Plast Reconstr Aesthet Surg. 2010 Jun;63(6):e525-8. doi: 10.1016/j.bjps.2010.01.033. Epub 2010 Feb 16.
The angiogenic induction property of the omentum makes it a promising pedicle for prefabricating flaps. Therefore, the objective of this paper is to establish the abdominal area to be prefabricated by the omental pedicle and to analyse the prefabricated potential (PP) according to the time delay between the pedicle introduction and the flap release.
Forty-four rabbits were divided into four groups (A, B, C and D). In group A, a piece of skin, subcutaneous tissue and abdominal cutaneous muscle was fully released and sutured again in place. In the other groups, a 9-cm2 omental pedicle containing the gastromental vessels distally tied was transposed and sutured to abdominal cutaneous muscle. A second procedure, consisting of incision and release of the flap that contained skin, subcutaneous and cutaneous abdominal muscle pediculated only by the omentum, was carried out. The only variation was the time delay between the two procedures: 7, 21 and 56 days for groups B, C and D, respectively. The flaps were inspected 15 days after the last procedure. The pieces of viable area were immunostained using anti-CD31 for estimation of the microvascular density.
The mean and maximum viable areas in group D were 45.29 and 99.37cm2, respectively (average PP=5.03 and maximum PP=11.04). There was no significant difference between the viable areas in groups C and D. The mean microvascular densities of groups B, C and D were 24.54, 33.20 and 27.03 vessels/mm2, respectively.
The omental tissue has great potential for prefabrication of flaps, and the delay time for the second procedure should be at least 21 days.
大网膜的血管生成诱导特性使其成为预制皮瓣的有前途的蒂。因此，本文的目的是确定由大网膜蒂预制的腹部区域，并根据蒂引入和皮瓣释放之间的时间延迟来分析预制潜力（PP）。
将 44 只兔子分为四组（A、B、C 和 D）。在 A 组中，充分释放并重新缝合一块皮肤、皮下组织和腹部皮肌。在其他组中，转移并缝合包含胃网膜血管的 9cm2 大网膜蒂，并用远端结扎。进行第二次手术，包括切开和释放仅由大网膜蒂供应的皮瓣，包含皮肤、皮下和腹部皮肌。唯一的变化是两次手术之间的时间延迟：B、C 和 D 组分别为 7、21 和 56 天。最后一次手术后 15 天检查皮瓣。使用抗 CD31 对有活力的区域进行免疫染色，以估计微血管密度。
D 组的平均和最大有活力面积分别为 45.29 和 99.37cm2（平均 PP=5.03，最大 PP=11.04）。C 组和 D 组的有活力面积无显著差异。B、C 和 D 组的平均微血管密度分别为 24.54、33.20 和 27.03 个血管/mm2。
大网膜组织具有预制皮瓣的巨大潜力，第二次手术的延迟时间应至少为 21 天。
