宿主蛋白与噬菌体 phi6 核心复合物相互作用以控制转录。
Interaction of a host protein with core complexes of bacteriophage phi6 to control transcription.
机构信息
Public Health Research Institute Center at UMDNJ, 225 Warren Street, Newark, NJ 07103, USA.
出版信息
J Virol. 2010 May;84(9):4821-5. doi: 10.1128/JVI.00026-10. Epub 2010 Feb 17.
Abstract
Bacteriophages of the family Cystoviridae have genomes consisting of three double-stranded RNA (dsRNA) segments, L, S, and M, packaged within a polyhedral capsid along with RNA polymerase. Transcription of genomic segment L is activated by the interaction of host protein YajQ with the capsid structure. Segment L codes for the proteins of the inner capsid, which are expressed early in infection. Green fluorescent protein (GFP) fusions with YajQ produce uniform fluorescence in uninfected cells and in cells infected with viruses not dependent on YajQ. Punctate fluorescence develops when cells are infected with YajQ-dependent viruses. It appears that the host protein binds to the infecting particles and remains with them during the entire infection period.
摘要
囊状噬菌体科的噬菌体具有由三个双链 RNA (dsRNA) 片段 L、S 和 M 组成的基因组，这些片段被包装在一个多面体衣壳内，同时还带有 RNA 聚合酶。基因组 L 片段的转录是通过宿主蛋白 YajQ 与衣壳结构的相互作用而激活的。L 片段编码衣壳内蛋白，这些蛋白在感染早期表达。与 YajQ 融合的绿色荧光蛋白 (GFP) 在未感染的细胞和不依赖 YajQ 的病毒感染的细胞中产生均匀的荧光。当细胞感染依赖 YajQ 的病毒时，会出现点状荧光。似乎宿主蛋白与感染颗粒结合，并在整个感染过程中与它们一起存在。
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