患者尿液中奥沙利铂的超快速 HPLC-ICP-MS 分析。
Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine.
机构信息
Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Applied Life Sciences, BOKU, Vienna, Muthgasse 18, 1190, Vienna, Austria.
出版信息
Anal Bioanal Chem. 2010 May;397(1):401-406. doi: 10.1007/s00216-010-3504-3. Epub 2010 Feb 19.
A novel method for rapid HPLC-ICP-MS analysis of oxaliplatin in human urine was developed implementing a stationary HPLC phase with a particle size of 1.8 microm. The method allowed a cycle time of <1 min at a HPLC flow rate of 0.9 mL min(-1). Procedural limits of detection of 0.05 microg L(-1) oxaliplatin (150 fg on column) were obtained. Analysis of oxaliplatin in patient urine showed that accurate quantification of the intact drug demanded for storage at -80 degrees C and rapid measurement after thawing.
建立了一种利用粒径为 1.8 微米的固定相高效液相色谱-电感耦合等离子体质谱法（HPLC-ICP-MS）快速检测人尿液中奥沙利铂的新方法。该方法在 HPLC 流速为 0.9mL/min 时，分析周期小于 1min。方法的检测限为 0.05μg/L（150fg 进样量）。对患者尿液中奥沙利铂的分析表明，为了准确测定完整药物的含量，需要在-80℃下储存，并在解冻后迅速进行测量。
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