Centro de Vigilancia Sanitaria Veterinaria, Universidad Complutense de Madrid, Spain.
Clin Microbiol Infect. 2010 Nov;16(11):1658-62. doi: 10.1111/j.1469-0691.2010.03198.x.
Some of the members of the Mycobacterium avium–intracellulare (MAI) complex are recognized as human pathogens in both immunocompromised and immunocompetent patients. The current molecular methods that are available for genotyping the MAI complex members can be both expensive and technically demanding. In this report, we describe for the first time the application of a real-time PCR and high-resolution melt approach to differentiate between the complex members by targeting a member of the Pro- Pro-Glu gene family, MACPPE24. To this end, reference strains of the M. avium subspecies and Mycobacterium intracellulare were used to optimize the technique. Then, this real-time PCR–high-resolution melt approach was used to distinguish ten M. avium ssp. hominissuis field isolates from the M. intracellulare reference strain.
一些鸟分枝杆菌-胞内分枝杆菌（MAI）复合体的成员被认为是免疫功能低下和免疫功能正常患者的人类病原体。目前用于对 MAI 复合体成员进行基因分型的分子方法既昂贵又技术要求高。在本报告中，我们首次描述了实时 PCR 和高分辨率熔解曲线分析方法的应用，该方法通过针对 Pro-Pro-Glu 基因家族的成员 MACPPE24 来区分复合体成员。为此，使用了鸟分枝杆菌亚种和胞内分枝杆菌的参考菌株来优化该技术。然后，使用该实时 PCR-高分辨率熔解曲线方法来区分 10 株从胞内分枝杆菌参考菌株分离出的人源鸟分枝杆菌亚种 hominissuis 田间分离株。
