抗体依赖性增强蛙病毒 3 感染。

Antibody dependent enhancement of frog virus 3 infection.

机构信息

Department of Biology, Trent University, Peterborough, ON, Canada.

出版信息

Virol J. 2010 Feb 18;7:41. doi: 10.1186/1743-422X-7-41.

DOI:10.1186/1743-422X-7-41

PMID:20167100

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2830962/

Abstract

BACKGROUND

Viruses included in the family Iridoviridae are large, icosahedral, dsDNA viruses that are subdivided into 5 genera. Frog virus 3 (FV3) is the type species of the genus Ranavirus and the best studied iridovirus at the molecular level. Typically, antibodies directed against a virus act to neutralize the virus and limit infection. Antibody dependent enhancement occurs when viral antibodies enhance infectivity of the virus rather than neutralize it.

RESULTS

Here we show that anti-FV3 serum present at the time of FV3 infection enhances infectivity of the virus in two non-immune teleost cell lines. We found that antibody dependent enhancement of FV3 was dependent on the Fc portion of anti-FV3 antibodies but not related to complement. Furthermore, the presence of anti-FV3 serum during an FV3 infection in a non-immune mammalian cell line resulted in neutralization of the virus. Our results suggest that a cell surface receptor specific to teleost cell lines is responsible for the enhancement.

CONCLUSIONS

This report represents the first evidence of antibody dependent enhancement in iridoviruses. The data suggests that anti-FV3 serum can either neutralize or enhance viral infection and that enhancement is related to a novel antibody dependent enhancement pathway found in teleosts that is Fc dependent.

摘要

背景

虹彩病毒科的病毒是大型二十面体双链 DNA 病毒，分为 5 个属。蛙病毒 3（FV3）是 Ranavirus 属的模式种，也是在分子水平上研究最多的虹彩病毒。通常情况下，针对病毒的抗体可起到中和病毒并限制感染的作用。而抗体依赖性增强（ADE）则是指病毒抗体增强了病毒的感染力，而非中和它。

结果

本研究表明，在 FV3 感染时存在的抗 FV3 血清可增强两种非免疫硬骨鱼类细胞系中病毒的感染力。我们发现，FV3 的 ADE 依赖于抗 FV3 抗体的 Fc 部分，而与补体无关。此外，在非免疫哺乳动物细胞系中 FV3 感染期间存在抗 FV3 血清会导致病毒被中和。我们的结果表明，特定于硬骨鱼类细胞系的细胞表面受体负责增强作用。