Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Mediators Inflamm. 2009;2009:426346. doi: 10.1155/2009/426346. Epub 2010 Jan 31.
The aim of this study is to analyze whether melatonin administration influenced the nuclear factor-kappa B (NF-kappaB) activity, proinflammatory cytokines expression, and oxidative response in the basilar artery after SAH. A total of 48 rabbits were randomly divided into four groups: control group, SAH group, SAH + vehicle group, and SAH + melatonin group. All SAH animals were subjected to injection of autologous blood into cisterna magna twice on day 0 and day 2. The melatonin was administered intraperitoneally at a dose of 5 mg/kg/12 h simultaneously with SAH from day 0 to day 5. The basilar arteries were extracted on day 5 after SAH. As a result, we found that vascular inflammation and oxidative stress were induced in all SAH animals. In animals given melatonin, basilar arterial NF-kappaB and pro-inflammatory cytokines were decreased in comparison to vehicle-treated animals. Measures of oxidative stress also showed significant downregulation after melatonin treatment. Furthermore, administration of melatonin prevented vasospasm on day 5 following SAH. In conclusion, post-SAH melatonin administration may attenuate inflammatory response and oxidative stress in the spasmodic artery, and this may be one mechanism involved in the therapeutic effect of melatonin on the subsequent vasospasm after SAH.
本研究旨在分析褪黑素给药是否影响蛛网膜下腔出血（SAH）后基底动脉的核因子-κB（NF-κB）活性、促炎细胞因子表达和氧化反应。将 48 只兔子随机分为四组：对照组、SAH 组、SAH+载体组和 SAH+褪黑素组。所有 SAH 动物均于第 0 天和第 2 天两次向枕大池内注射自体血。从第 0 天到第 5 天，同时给予褪黑素 5mg/kg/12h 腹腔内给药。SAH 后第 5 天提取基底动脉。结果发现，所有 SAH 动物均诱导血管炎症和氧化应激。与载体处理的动物相比，给予褪黑素的动物基底动脉 NF-κB 和促炎细胞因子减少。氧化应激的测量值在褪黑素治疗后也显示出显著下调。此外，褪黑素给药可预防 SAH 后第 5 天的血管痉挛。总之，SAH 后褪黑素给药可能减轻痉挛动脉的炎症反应和氧化应激，这可能是褪黑素对 SAH 后随后发生的血管痉挛的治疗作用的一种机制。
