Center for Scientific Research, San Pietro Hospital Fatebenefratelli, Rome, Italy.
Gynecol Endocrinol. 2010 Jun;26(6):435-9. doi: 10.3109/09513591003632076.
To test the hypothesis that serum or intrafollicular concentrations of adrenomedullin (AM) would correlate with reproductive outcomes in in vitro fertilisation (IVF) cycles.
Serum and follicular fluid samples were collected during transvaginal oocyte retrieval. The follicular fluid was individually aspirated, and the presence of oocyte was recorded. AM concentrations were measured using an enzyme-linked immunosorbent assay.
Department of Gynaecology, Perinatology and Child Health, 'Sapienza' University of Rome, Italy.
Eighty women undergoing IVF for primary infertility aged 18-45 years.
AM concentrations in plasma and follicular fluid were correlated to follicular fluid volume, presence of oocyte, oocyte maturation, embryo grading, fertilisation and pregnancy rates, live-birth rate and plasma estrogen concentration.
Monofollicular fluid AM concentrations did not differ between follicles containing oocyte and those without oocyte; however, AM concentrations were lower in follicles that resulted in pregnancy than in those that failed. Serum but not follicular fluid AM concentrations correlated with serum estrogen levels. Follicular fluid AM correlated with plasma AM levels.
We conclude that higher level of AM in the follicular fluid appears to be associated with a negative outcome in IVF treatment.
检验血清或卵泡液中肾上腺髓质素（AM）浓度与体外受精（IVF）周期中生殖结局相关的假说。
在经阴道取卵过程中采集血清和卵泡液样本。逐个抽吸卵泡液，并记录卵母细胞的存在情况。采用酶联免疫吸附试验测定 AM 浓度。
意大利罗马“萨皮恩扎”大学妇科、围产儿和儿童健康系。
80 名年龄在 18-45 岁之间的原发性不孕行 IVF 治疗的女性。
血浆和卵泡液中的 AM 浓度与卵泡液体积、卵母细胞存在、卵母细胞成熟、胚胎分级、受精和妊娠率、活产率以及血浆雌激素浓度相关。
含有卵母细胞的卵泡和不含卵母细胞的卵泡之间的单卵泡液 AM 浓度没有差异；然而，妊娠的卵泡中的 AM 浓度低于未妊娠的卵泡。血清 AM 浓度而非卵泡液 AM 浓度与血清雌激素水平相关。卵泡液 AM 与血浆 AM 水平相关。
我们得出结论，卵泡液中较高水平的 AM 似乎与 IVF 治疗的不良结局相关。
