Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, Canada V0H 1Z0.
J Invertebr Pathol. 2010 Jun;104(2):105-9. doi: 10.1016/j.jip.2010.02.006. Epub 2010 Feb 19.
Last-instar larvae of the western cherry fruit fly, Rhagoletis indifferens, were subjected to Beauveria bassiana GHA incorporated into sterile sand and non-sterile orchard soil. Mycosis in the pupal stage was observed in >20% of buried R. indifferens pupae and >80% of larvae entering sand treated with either of two B. bassiana isolates. When pre-pupal larvae burrowed into conidium-treated non-sterile cherry orchard soil, the incidence of mycosis, on both the puparia and internally developing pupae, increased with dose. Internal pupal tissues were found to contain B. bassiana. Increasing the soil moisture level from 20% to 35% water holding capacity did not have an effect on the percentage of mycosed pupae. This is the first evidence that the preimaginal stages of R. indifferens are susceptible to infection by B. bassiana.
晚期幼虫的西方樱桃实蝇，Rhagoletis indifferens，受到了球孢白僵菌 GHA 掺入无菌沙和非无菌果园土壤。在 >20%的被掩埋的 R. indifferens 蛹和 >80%的进入用两种白僵菌分离株处理的沙中的幼虫中观察到蛹期的真菌病。当预蛹幼虫钻入含有分生孢子的处理过的非无菌樱桃果园土壤时，真菌病的发生率，无论是在蛹壳上还是内部发育的蛹中，都随着剂量的增加而增加。内部蛹组织中含有白僵菌。将土壤水分水平从 20%增加到 35%的持水能力并没有影响真菌病蛹的百分比。这是第一个证据表明，R. indifferens 的预蛹阶段易受球孢白僵菌的感染。
