Division of Marine Environment & Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea.
Comp Biochem Physiol A Mol Integr Physiol. 2010 Jun;156(2):262-8. doi: 10.1016/j.cbpa.2010.02.013. Epub 2010 Feb 19.
We determined oxidative stress by measuring the expression and activity of 3 antioxidant enzymes [Cu/Zn-superoxide dismutase (Cu/Zn-SOD), catalase (CAT) and glutathione peroxidase (GPX)] in black porgy exposed to thermal (20 degrees C-->30 degrees C) and hypoosmotic (35 psu-->10 psu and 0 psu) stresses. The expression and activity of antioxidant enzymes were significantly higher after exposure to 30 degrees C, 10 psu, and 0psu. Furthermore, we measured H(2)O(2) and lipid peroxidation (LPO) levels. As a result, H(2)O(2) and LPO levels were significantly increased after exposure to thermal (20 degrees C-->30 degrees C) and hypoosmotic stress (35 psu-->10 psu and 0 psu) stress. These results indicate that thermal and hypoosmotic stress induces oxidative stress in black porgy. Additionally, we investigated the changes due to thermal and hypoosmotic stress by measuring plasma cortisol and ion (Na(+) and Cl(-)) levels. Plasma cortisol levels increased at 30 degrees C and at 10 psu and then decreased at 0 psu. However, plasma Na(+) and Cl(-) levels did not change after exposure to thermal stress (30 degrees C), and decreased at 10 psu and 0 psu. In conclusion, thermal and hypoosmotic environments increase oxidative stress, thereby these results may be indicators of oxidative stress in black porgy.
我们通过测量三种抗氧化酶（Cu/Zn-超氧化物歧化酶（Cu/Zn-SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX））的表达和活性来确定氧化应激。在热（20°C-30°C）和低渗（35 psu-10 psu 和 0 psu）应激下暴露的黑鲷中，抗氧化酶的表达和活性显著升高。此外，我们测量了 H2O2 和脂质过氧化（LPO）水平。结果表明，在热（20°C-30°C）和低渗应激（35 psu-10 psu 和 0 psu）下，H2O2 和 LPO 水平显著升高。这些结果表明，热和低渗应激会导致黑鲷产生氧化应激。此外，我们通过测量血浆皮质醇和离子（Na+和 Cl-）水平来研究热和低渗应激引起的变化。血浆皮质醇水平在 30°C 和 10 psu 时升高，然后在 0 psu 时降低。然而，在热应激（30°C）下，血浆 Na+和 Cl-水平没有变化，在 10 psu 和 0 psu 时降低。总之，热和低渗环境会增加氧化应激，因此这些结果可能是黑鲷氧化应激的指标。
