Department of Otorhinolaryngology-Head and Neck Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
Otolaryngol Head Neck Surg. 2010 Mar;142(3):332-7. doi: 10.1016/j.otohns.2009.10.050. Epub 2010 Jan 8.
To evaluate the expression of cell-cycle regulators in papillary thyroid carcinoma in relation to lymph node metastatic features, and to determine whether immunohistochemical staining of cell-cycle markers can predict lymph node metastasis.
Cross-sectional study of prior surgical specimens.
Academic tertiary referral center.
We reviewed the clinical records of patients who had undergone surgery for thyroid cancer and follicular adenoma between January 2005 and May 2008 at our clinic. Among these cases, 92 patients, comprising 28 patients with follicular adenoma, 32 with papillary thyroid carcinoma without lymph node metastasis, and 32 with papillary thyroid carcinoma with lymph node metastasis, were selected randomly. Formalin-fixed, paraffin-embedded tissues from the 92 patients were immunohistochemically stained for cyclin D1, cyclin E, p27(kip1), and p57(kip2), and protein expression levels were quantified and compared among the groups.
Tumor specimens from the papillary thyroid carcinoma group had significantly higher expression levels of cyclin D1 and cyclin E, and cytoplasmic expression of p57(kip2) than the other two groups (P < 0.05). In particular, all malignant cases expressed cyclin D1, and cytoplasmic p57(kip2) was expressed only in malignant cases. Furthermore, differences in the grade of cyclin D1 expression according to lymph node metastasis were statistically significant (P < 0.05).
Our results suggest that immunohistochemistry of certain cell-cycle regulators may be helpful in the diagnosis of papillary thyroid carcinoma, and that cyclin D1 in particular may be a useful marker for evaluating lymph node metastasis.
评估细胞周期调控因子在甲状腺乳头状癌中的表达与淋巴结转移特征的关系，并确定细胞周期标志物的免疫组织化学染色是否可以预测淋巴结转移。
回顾性分析 2005 年 1 月至 2008 年 5 月在我院接受手术治疗的甲状腺癌和滤泡性腺瘤患者的临床资料。选择其中 92 例患者作为研究对象，包括 28 例滤泡性腺瘤患者、32 例无淋巴结转移的甲状腺乳头状癌患者和 32 例有淋巴结转移的甲状腺乳头状癌患者。对 92 例患者的福尔马林固定、石蜡包埋组织进行细胞周期蛋白 D1、细胞周期蛋白 E、p27（kip1）和 p57（kip2）的免疫组织化学染色，并对各组的蛋白表达水平进行定量和比较。
与另外两组相比，甲状腺乳头状癌组的肿瘤标本中 cyclin D1 和 cyclin E 的表达水平以及 p57（kip2）的细胞质表达水平显著升高（P<0.05）。特别是所有恶性病例均表达 cyclin D1，而仅在恶性病例中表达细胞质 p57（kip2）。此外，根据淋巴结转移情况，cyclin D1 表达程度的差异具有统计学意义（P<0.05）。
我们的研究结果表明，某些细胞周期调控因子的免疫组织化学染色可能有助于甲状腺乳头状癌的诊断，特别是 cyclin D1 可能是评估淋巴结转移的有用标志物。
