钙内流阻滞剂在人中性粒细胞中的作用支持受体操纵性钙内流的作用。
Actions of calcium influx blockers in human neutrophils support a role for receptor-operated calcium entry.
机构信息
Leukocyte and Ion Channel Research Laboratory, School of Health and Biosciences, University of East London, Stratford Campus, Romford Road, London E15 4LZ, UK.
出版信息
Cell Immunol. 2010;262(1):6-10. doi: 10.1016/j.cellimm.2010.02.001. Epub 2010 Feb 4.
The action of two potent store operated Ca2+ entry (SOCE) inhibitors, ML-9 and GdCl3 on Ca2+ fluxes induced by the pro-inflammatory agonists FMLP, PAF, LTB(4) as well as the receptor-independent stimulus thapsigargin has not been documented in human neutrophils. In this study, ML-9 enhanced both release and subsequent Ca2+ influx in response to agonists whereas it enhanced Ca2+ release by thapsigargin, but inhibited Ca2+ influx. In contrast, 1muM GdCl3 completely inhibited Ca2+ influx in response to thapsigargin, but only partially blocked Ca2+ influx after agonist stimulation. These results strongly suggest a major role for receptor-operated Ca2+ influx in human neutrophils.
尚未在人中性粒细胞中记录到两种强效的储存操作钙（Ca2+）内流（SOCE）抑制剂 ML-9 和 GdCl3 对促炎激动剂 FMLP、PAF、LTB4 以及受体非依赖性刺激物 thapsigargin 诱导的 Ca2+流的作用。在这项研究中，ML-9 增强了对激动剂的释放和随后的 Ca2+内流，而增强了 thapsigargin 的 Ca2+释放，但抑制了 Ca2+内流。相比之下，1μM GdCl3 完全抑制了 thapsigargin 引起的 Ca2+内流，但仅部分阻断了激动剂刺激后的 Ca2+内流。这些结果强烈表明受体操作的 Ca2+内流在人中性粒细胞中起主要作用。
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