Nrf2的下调增强了奥沙利铂对H460细胞的细胞毒性
[Down-regulation of Nrf2 enhances the cytotoxicity of Oxaliplatin to H460 cells].
作者信息
Xin Ai, Ren Hua-yu, Tang Xiu-wen
机构信息
Department of Biochemistry and Genetics, College of Medicine, Zhejiang University, Hangzhou, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 Jan;39(1):11-6. doi: 10.3785/j.issn.1008-9292.2010.01.003.
OBJECTIVE
To establish a stable H460 cell line with Nrf2 down-regulation to study the role of Nrf2 in Oxaliplatin resistance.
METHODS
Nrf2 down-regulated H460 cell line was obtained by transfecting cells with pRS-hNrf2 and followed by screening for Nrf2 expression by Western blotting. The cell lines were further characterized by analysing cellular GSH levels and cytotoxicity to anti-cancer drugs with MTS.
RESULT
Nrf2 down-regulated H460 cell lines were established successfully. Cellular GSH level reduced significantly in the H460-C9 and H460-C13 compared to control cells (P(C9)= 0.00249, P(C13)= 0.03944). Down-regulation of Nrf2 in H460 sensitized the anti-cancer effect of Oxaliplatin and Doxorubicin.
CONCLUSION
Nrf2 plays an important role in drug resistance. Down regulation of Nrf2 in H460 cell enhances cytotoxicity of Oxaliplatin.
目的
建立Nrf2表达下调的稳定H460细胞系，以研究Nrf2在奥沙利铂耐药中的作用。
方法
用pRS-hNrf2转染细胞，通过蛋白质免疫印迹法筛选Nrf2表达，从而获得Nrf2表达下调的H460细胞系。通过分析细胞内谷胱甘肽（GSH）水平以及用MTS法检测细胞对抗癌药物的细胞毒性，对细胞系进行进一步鉴定。
结果
成功建立了Nrf2表达下调的H460细胞系。与对照细胞相比，H460-C9和H460-C13细胞内的GSH水平显著降低（P(C9)=0.00249，P(C13)=0.03944）。H460细胞中Nrf2的下调增强了奥沙利铂和阿霉素的抗癌效果。
结论
Nrf2在耐药中起重要作用。H460细胞中Nrf2的下调增强了奥沙利铂的细胞毒性。
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