Ogle C K, Wu J Z, Alexander J W, Fischer J E, Ogle J D
Shriners Burns Institute, Cincinnati, OH 45219.
Prostaglandins. 1991 Feb;41(2):169-83. doi: 10.1016/0090-6980(91)90029-f.
It was the purpose of this study to determine the effects of the in vivo administration of endotoxin on certain in vitro hepatocyte and Kupffer cell functions. An Alzet osmotic pump that contained endotoxin (LPS, 2.5 mg/100g) was implanted into the peritoneal cavity of 300g guinea pigs and delivered the endotoxin over a period of four days. In vivo administration of LPS did not cause a change in the in vitro release of albumin by isolated hepatocytes. However, when hepatocytes were co-cultured with Kupffer cells there was a significant decrease in albumin release for both control and LPS-treated animals. There was no difference between control and LPS-treated animals in the release of C3 by hepatocytes. However, there was a significant increase over the control group in C3 release by Kupffer cells from LPS-treated animals. When hepatocytes and Kupffer cells were cultured together, their release of C3 was not additive. Kupffer cells from LPS-treated animals released significantly greater amounts of PGE2 than control animals when stimulated in vitro with LPS. Thus, these Kupffer cells appeared to be primed to respond to an in vitro challenge of LPS. Kupffer cells from LPS-treated animals had significantly depressed antibody dependent cellular cytotoxicity (ADCC). This endotoxin model is useful for determining the in vivo effects of endotoxin on cellular function and gives some indirect evidence for the detrimental effects of LPS on the immune system and host defense.
本研究的目的是确定体内给予内毒素对某些体外肝细胞和库普弗细胞功能的影响。将含有内毒素（脂多糖，2.5mg/100g）的Alzet渗透泵植入300g豚鼠的腹腔，并在四天内递送内毒素。体内给予脂多糖并未导致分离的肝细胞体外白蛋白释放发生变化。然而，当肝细胞与库普弗细胞共培养时，对照动物和脂多糖处理动物的白蛋白释放均显著降低。肝细胞释放C3方面，对照动物和脂多糖处理动物之间没有差异。然而，脂多糖处理动物的库普弗细胞释放的C3比对照组显著增加。当肝细胞和库普弗细胞一起培养时，它们释放的C3不是相加的。用脂多糖体外刺激时，脂多糖处理动物的库普弗细胞释放的PGE2比对照动物显著更多。因此，这些库普弗细胞似乎已被激活以应对脂多糖的体外刺激。脂多糖处理动物的库普弗细胞的抗体依赖性细胞毒性（ADCC）显著降低。这种内毒素模型对于确定内毒素对细胞功能的体内影响很有用，并为脂多糖对免疫系统和宿主防御的有害影响提供了一些间接证据。
