Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chuoh-ku, Chiba, Japan.
Cancer Sci. 2010 Jan;101(1):289-91. doi: 10.1111/j.1349-7006.2009.01381.x.
We detected adenoviral DNA fragments in excretions of 10 esophageal cancer patients by DNA-PCR after tumor injection of Ad-CMV-vector. A total of 220 samples consisting of feces, gargling saliva, urine, and blood plasma were assessed. A total of 29.7% of feces samples and 13.2% of gargling saliva samples were positive for adenoviral DNA fragments, but 89.7% of the positive feces samples and all of the positive gargling saliva samples turned negative on day 12 after tumor injection. Although adenoviral DNA fragments may be pathogen-free, patients' feces and gargling saliva contain adenoviral DNA fragments for 12 days after injection.
我们通过肿瘤内注射 Ad-CMV-vector 后对 10 例食管癌患者的排泄物进行 DNA-PCR 检测到腺病毒 DNA 片段。共评估了 220 例粪便、漱口唾液、尿液和血浆样本。粪便样本的腺病毒 DNA 片段阳性率为 29.7%,漱口唾液样本的阳性率为 13.2%,但肿瘤注射后 12 天,89.7%的阳性粪便样本和所有的阳性漱口唾液样本均转为阴性。虽然腺病毒 DNA 片段可能是无病原体的,但患者的粪便和漱口唾液在注射后 12 天内仍含有腺病毒 DNA 片段。