NSF 独立融合含沙门氏菌的晚期吞噬体与早期内体。

NSF independent fusion of Salmonella-containing late phagosomes with early endosomes.

机构信息

National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.

出版信息

FEBS Lett. 2010 Mar 19;584(6):1251-6. doi: 10.1016/j.febslet.2010.02.040. Epub 2010 Feb 20.

DOI:10.1016/j.febslet.2010.02.040

PMID:20176016

Abstract

Initial characterizations of live-Salmonella-containing early (LSEP) and late phagosomes (LSLP) in macrophages show that both phagosomes retain Rab5 and EEA1. In addition, LSEP specifically contain transferrin receptor whereas LSLP possess relatively more rabaptin-5. In contrast to LSLP, late-Salmonella-containing vacuoles in epithelial cells show significantly reduced levels of Rab5 and EEA1. Subsequent results demonstrate that both phagosomes efficiently fuse with early endosomes (EE). In contrast to LSEP, fusion between LSLP and EE is insensitive to ATPgammaS treatment. Furthermore, LSLP fuses with EE in absence of NEM-sensitive fusion factor (NSF) as well as in the presence of NSF:D1EQ mutant demonstrating that LSLP fusion with EE is NSF independent.

摘要

巨噬细胞中活 Salmonella 包含的早期吞噬体（LSEP）和晚期吞噬体（LSLP）的初步特征表明，两种吞噬体都保留 Rab5 和 EEA1。此外，LSEP 特异性包含转铁蛋白受体，而 LSLP 则具有相对较多的 rabaptin-5。与 LSLP 相反，上皮细胞中含有晚期 Salmonella 的液泡显示 Rab5 和 EEA1 的水平显著降低。随后的结果表明，两种吞噬体都能有效地与早期内体（EE）融合。与 LSEP 相反，LSLP 与 EE 之间的融合对 ATPgammaS 处理不敏感。此外，LSLP 在缺乏 NEM 敏感融合因子（NSF）以及存在 NSF:D1EQ 突变体的情况下与 EE 融合，这表明 LSLP 与 EE 的融合不依赖于 NSF。