Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR China.
J Ethnopharmacol. 2010 May 4;129(1):5-9. doi: 10.1016/j.jep.2010.02.002. Epub 2010 Feb 20.
Modified Si-Miao-San (mSMS) is a prescription modified from Si-Miao-San which is an ancient Chinese prescription used to treat various ailments.
Modified Si-Miao-San (mSMS) has been used for the treatment of infectious and inflammatory disorders in the clinic. This study was aimed to investigate its anti-inflammatory activity and underlying mechanism at cellular and molecular levels.
We stimulated RAW264.7 cells with Lipopolysaccharide (LPS) and observed effects of mSMS on the release of inflammatory mediators such as: tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), NO, and relative gene expressions. Meanwhile, we also investigated the modulation of mSMS in inflammatory signal transduction mediated through extracellular signal-regulated protein kinase (ERK) and nuclear factor-kappaB (NF-kappaB) pathway.
Our findings demonstrated that mSMS significantly inhibited the excessive production of NO, TNF-alpha and IL-6 and the over expression of relative genes in LPS-stimulated macrophages. In addition, mSMS suppressed LPS-induced ERK1/2-phosphorylation and inhibited the activation of NF-kappaB by attenuation of I kappaB-alpha degradation.
Our results suggest that the anti-inflammatory properties of mSMS might result from the inhibition of inflammatory mediators, such as NO, TNF-alpha and IL-6, via suppression of ERK and NF-kappaB dependent pathways.
改良四妙散（mSMS）是从四妙散（一种用于治疗各种疾病的古老中药方）改良而来的处方。
改良四妙散（mSMS）已在临床上用于治疗感染和炎症性疾病。本研究旨在从细胞和分子水平上研究其抗炎活性及其潜在机制。
我们用脂多糖（LPS）刺激 RAW264.7 细胞，观察 mSMS 对炎症介质释放的影响，如肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、NO 和相对基因表达。同时，我们还研究了 mSMS 通过细胞外信号调节激酶（ERK）和核因子-κB（NF-κB）途径对炎症信号转导的调节作用。
我们的研究结果表明，mSMS 能显著抑制 LPS 刺激的巨噬细胞中 NO、TNF-α和 IL-6 的过度产生和相对基因的过度表达。此外，mSMS 通过抑制 IκB-α降解，抑制 LPS 诱导的 ERK1/2 磷酸化，并抑制 NF-κB 的激活。
我们的研究结果表明，mSMS 的抗炎作用可能是通过抑制炎症介质，如 NO、TNF-α和 IL-6，通过抑制 ERK 和 NF-κB 依赖的途径来实现的。
