Laboratoire de Biochimie & Biologie Moléculaire, CRSBAN/UFR/SVT, Université de Ouagadougou 03 BP 7021 Ouagadougou 03, Burkina Faso.
Virol J. 2010 Feb 23;7:48. doi: 10.1186/1743-422X-7-48.
Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD.
秋葵叶卷曲病（OLCD）是秋葵（Abelmoschus esculentus）生产的主要限制因素，在非洲广泛存在。利用大量代表布基纳法索（BF）主要种植区的样本，我们表明该疾病与单分体伴生病毒和卫星 DNA 复合物有关。与 OLCD 相关的 23 个棉叶卷曲 Gezira 病毒（CLCuGV）分离株的完整基因组序列，核苷酸同一性为 95%至 99%，已被克隆和测序。还对 6 个贝塔卫星和 4 个阿尔法卫星（DNA-1）分子进行了特征描述。与 CLCuGV 分离株相关的 6 个贝塔卫星分离株对应于棉叶卷曲 Gezira 贝塔卫星（CLCuGB）（88%至 98%核苷酸同一性）。一种阿尔法卫星分离株是棉叶卷曲 Gezira 阿尔法卫星（CLCuGA）的变体（89%核苷酸同一性），而其他三个分离株似乎对应于一种新的阿尔法卫星（CLCuGA 最相似序列为 52%至 60%核苷酸同一性），暂命名为布基纳法索秋葵卷曲阿尔法卫星（OLCBFA）。病毒的重组分析表明，所有 CLCuGV 分离株都具有种间重组的起源，其亲本与霍利霍克叶卷曲病毒（AY036009）和番茄叶卷曲戴安娜病毒（AM701765）密切相关。结合卫星 DNA 的存在，这些结果突出了与 OLCD 相关的伴生病毒的复杂性。
