Chen Mei-Yuan, Yan Shu-Chao, Yin Chun-Ping, Ye Lei, Zhang Meng-Ke, Yang Jun, Liu Ji-Hong
Department of Urology, Tongji Hospital Affiliated to Tongli Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2010 Jan;16(1):34-9.
To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia (BPH) stromal cells.
We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5, 25, 50 and 100 microg/ml, and established a PBS blank control, a dimethyl sulphoxide (DMSO) negative control and four finasteride positive control groups (at the concentration of 12.5, 25.0, 50.0 and 100.0 microg/ml). We determined the effects of different concentrations of red clover isoflavones on the proliferation of the cells by MTT assay and on their apoptosis by Annexin V/PI double staining flow cytometry.
Red clover isoflavones inhibited the proliferation of the BPH stromal cells by 18.86% at 25.0 microg/ml, compared with 5.17% in the blank control group (P < 0.05), and more obviously at a higher concentration. At 50.0 microg/ml, red clover isoflavones exhibited a weaker inhibitory effect than finasteride (28% vs 69.88% , P < 0.05). Annexin V/PI double staining flow cytometry showed that red clover isoflavones at 25.0 microg/ml induced the apoptosis of the prostate stromal cells by (18.54 +/- 2.5)%, with significant differences from the negative control and blank control (P < 0.01).
Red clover isoflavones can inhibit the proliferation and promote the apoptosis of human BPH stromal cells.
评估红三叶草异黄酮对人良性前列腺增生（BPH）基质细胞增殖和凋亡的影响。
我们用浓度为12.5、25、50和100微克/毫升的红三叶草异黄酮处理人前列腺基质细胞，并设立PBS空白对照组、二甲基亚砜（DMSO）阴性对照组和四个非那雄胺阳性对照组（浓度分别为12.5、25.0、50.0和100.0微克/毫升）。我们通过MTT法测定不同浓度红三叶草异黄酮对细胞增殖的影响，并通过Annexin V/PI双染流式细胞术测定其对细胞凋亡的影响。
与空白对照组的5.17%相比，25.0微克/毫升的红三叶草异黄酮使BPH基质细胞的增殖受到18.86%的抑制（P<0.05），且在更高浓度下抑制作用更明显。在50.0微克/毫升时，红三叶草异黄酮的抑制作用比非那雄胺弱（28%对69.88%，P<0.05）。Annexin V/PI双染流式细胞术显示，25.0微克/毫升的红三叶草异黄酮诱导前列腺基质细胞凋亡（18.54±2.5）%，与阴性对照组和空白对照组有显著差异（P<0.01）。
红三叶草异黄酮可抑制人BPH基质细胞的增殖并促进其凋亡。
