Zhang Qing-Yun, Mo Zeng-Nan, Liu Xi-Di
Research Institute of Urology, Guangxi Medical University, Nanning , Guangxi 530021, China.
Zhonghua Nan Ke Xue. 2010 Jan;16(1):84-8.
To investigate the effects of curcumin on the expressions of TNF-alpha, IL-6 and IL-8 in rats with chronic nonbacterial prostatitis.
Sixty healthy adult male SD rats with the body weight of 200 -220 g were equally and randomly divided into a normal control, a positive control, a model, an oral curcumin and an intraperitoneal curcumin group. The rat models of chronic nonbacterial prostatitis were made by hypodermic injection of estradiol benzoate at the dose of 0.25 mg/(kg x d) for 30 days after castration, and then treated with curcumin at 200 mg/(kg x d) by gavage or intraperitoneal injection. The positive controls received oral celebrex at 250 mg/(kg x d), while the normal control and model groups were given saline by gavage. After a week of treatment, the levels of TNF-alpha, IL-6 and IL-8 in the serum and prostate tissues of the rats were detected by ELISA assay.
The levels of TNF-alpha and IL-8 in the serum and prostate tissues were significantly lower in the intraperitoneal curcumin than in the positive control group (P < 0.05), but the expression of IL-6 showed no significant difference between the two groups (P > 0.01).
Curcumin is efficacious for chronic nonbacterial prostatitis in rats, and the action mechanism may be associated with its decreasing effect on the proinflammatory cytokines IL-8 and TNF-alpha in the blood and tissues.
探讨姜黄素对慢性非细菌性前列腺炎大鼠肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）表达的影响。
将60只体重200 - 220 g的健康成年雄性SD大鼠平均随机分为正常对照组、阳性对照组、模型组、姜黄素口服组和姜黄素腹腔注射组。采用去势后皮下注射苯甲酸雌二醇0.25 mg/(kg·d)，连续30天的方法制备慢性非细菌性前列腺炎大鼠模型，然后分别给予200 mg/(kg·d)姜黄素灌胃或腹腔注射。阳性对照组给予塞来昔布250 mg/(kg·d)口服，正常对照组和模型组给予生理盐水灌胃。治疗1周后，采用酶联免疫吸附测定（ELISA）法检测大鼠血清和前列腺组织中TNF-α、IL-6和IL-8的水平。
姜黄素腹腔注射组大鼠血清和前列腺组织中TNF-α和IL-8水平显著低于阳性对照组（P < 0.05），但两组IL-6表达差异无统计学意义（P > 0.01）。
姜黄素对大鼠慢性非细菌性前列腺炎有效，其作用机制可能与其降低血液和组织中促炎细胞因子IL-8和TNF-α的水平有关。
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