气相色谱法研究硝基乙烷和硝基乙醇对猪粪便中细菌吲哚和 3-甲基吲哚（粪臭素）生成的影响。

Effect of nitroethane and nitroethanol on the production of indole and 3-methylindole (skatole) from bacteria in swine feces by gas chromatography.

机构信息

U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, Texas 77845-4988, USA.

出版信息

J Environ Sci Health B. 2009 Aug;44(6):613-20. doi: 10.1080/03601230903000701.

DOI:10.1080/03601230903000701

PMID:20183070

Abstract

Indole and 3-methylindole (skatole) are odor pollutants in livestock waste, and skatole is a major component of boar taint. Skatole causes pulmonary edema and emphysema in ruminants and causes damage to lung Clara cells in animals and humans. A gas chromatographic method that originally used a nitrogen-phosphorus detector to increase sensitivity was modified resulting in an improved flame ionization detection response for indole and skatole of 236% and 207%, respectively. The improved method eliminates the large amount of indole decomposition in the injector. A 10 micro g mL(-1) spike of indole and skatole in water and swine fecal slurries resulted in recovery of 78.5% and 96% in water and 76.1% and 85.8% in fecal slurries, respectively. The effect of the addition of nitroethane and nitroethanol at 21.8 mM in swine fecal slurries was studied on the microbial production of indole and skatole. Nitroethane and nitroethanol decreased the production of skatole in swine fecal slurries at 24 h. The nitroethane effect on l-tryptophan-supplemented fecal slurries after 6 and 24 h incubation resulted in a decrease of 69.0% (P = 0.02) and 23.5% skatole production, respectively, and a decrease of 14.9% indole at 6 h, but an increase in indole production of 81.1% at 24 h.

摘要

吲哚和 3-甲基吲哚（粪臭素）是家畜废物中的气味污染物，粪臭素是公猪异味的主要成分。粪臭素会导致反刍动物肺水肿和肺气肿，并对动物和人类的肺 Clara 细胞造成损害。一种原本使用氮磷检测器来提高灵敏度的气相色谱方法进行了改进，从而使吲哚和粪臭素的火焰离子化检测响应分别提高了 236%和 207%。改进后的方法消除了进样器中大量吲哚的分解。在水中和猪粪便悬浮液中加入 10 μg mL(-1)的吲哚和粪臭素，在水中的回收率分别为 78.5%和 96%，在粪便悬浮液中的回收率分别为 76.1%和 85.8%。研究了在猪粪便悬浮液中添加 21.8 mM 的硝基乙烷和硝基乙醇对吲哚和粪臭素微生物生成的影响。硝基乙烷和硝基乙醇降低了猪粪便悬浮液中粪臭素的生成。硝基乙烷对补充 l-色氨酸的粪便悬浮液在 6 和 24 h 孵育后的影响分别导致粪臭素的生成减少了 69.0%（P = 0.02）和 23.5%，6 h 时吲哚减少 14.9%，但 24 h 时吲哚生成增加了 81.1%。