Department of Environmental Engineering, Istanbul Technical University, Istanbul, Turkey.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Dec;44(14):1551-6. doi: 10.1080/10934520903263470.
The aim of this study was to determine the effect of toluene on an anaerobic sludge taken from a full-scale upflow anaerobic sludge blanket (UASB) reactor in terms of potential activity and composition of acetoclastic methanogens. Specific methanogenic activity (SMA) test results showed that 5%, 9.5%, 14%, 24%, 29%, 38% and 62% inhibition occurred in the potential methane production (PMP) rate of the sludge at toluene concentrations of 0.1 mM, 0.2 mM, 0.3 mM, 0.4 mM, 0.5 mM, 0.6 mM and 1 mM, respectively. Fluorescence in situ hybridization (FISH) results showed that relative abundance of archaeal cells was approx. 19% throughout the SMA tests. The anaerobic sludge was dominated by acetoclastic genus Methanosaeta which were slightly affected by increasing toluene concentrations do not have any effect on relative abundance of Methanosaeta spp., which was between 73% +/- 1.6 and 68% +/- 2.1 of the archaeal population.
本研究旨在探讨甲苯对取自规模为上流式厌氧污泥床（UASB）反应器的厌氧污泥的影响，特别是对产乙酸甲烷菌的潜在活性和组成的影响。特异性甲烷活性（SMA）测试结果表明，在甲苯浓度分别为 0.1mM、0.2mM、0.3mM、0.4mM、0.5mM、0.6mM 和 1mM 时，污泥的潜在甲烷生成（PMP）速率分别出现了 5%、9.5%、14%、24%、29%、38%和 62%的抑制。荧光原位杂交（FISH）结果表明，在 SMA 测试过程中，古菌细胞的相对丰度约为 19%。厌氧污泥主要由产乙酸甲烷菌属 Methanosaeta 主导，随着甲苯浓度的增加，它们受到的轻微影响并未对 Methanosaeta spp. 的相对丰度产生任何影响，该菌属在古菌群体中的比例为 73% +/- 1.6%至 68% +/- 2.1%。
