钌（II）多吡啶配合物与DNA的DNA结合和光切割特性及其对真核微生物的毒性研究。
DNA-binding and photocleavage properties of Ru(II) polypyridyl complexes with DNA and their toxicity studies on eukaryotic microorganisms.
作者信息
Reddy Kotha Laxma, Reddy Y Harish Kumar, Satyanarayana S
机构信息
Department of Chemistry, Osmania University, Hyderabad, India.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2009 Oct;28(10):953-68. doi: 10.1080/15257770903307060.
Four Ru(II) polypyridyl complexes, Ru(bpy)(2)(7-NO(2)-dppz), Ru(bpy)(2)(7-CH(3)-dppz), Ru(phen)(2)(7-NO(2)-dppz), and Ru(phen)(2)(7-CH(3)-dppz) (bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline), (7-Nitro-dppz = 7-Nitro dipyrido[3,2-a:2'-3'-c]phenazine, 7-CH(3)-dppz = 7-Methyl dipyrido[3,2-a:2'-3'-c]phenazine), have been synthesized and characterized by IR, UV, elemental analysis, (1)H NMR, (13)C-NMR, and mass spectroscopy. The DNA-binding properties of the four complexes were investigated by spectroscopic and viscosity measurements. The results suggest that all four complexes bind to DNA via an intercalative mode. Under irradiation at 365 nm, all four complexes were found to promote the photocleavage of plasmid pBR 322 DNA. Toxicological effects of the selected complexes were performed on industrially important yeasts (eukaryotic microorganisms).
已合成了四种钌（II）多吡啶配合物，即Ru(bpy)(2)(7-NO(2)-dppz)、Ru(bpy)(2)(7-CH(3)-dppz)、Ru(phen)(2)(7-NO(2)-dppz)和Ru(phen)(2)(7-CH(3)-dppz)（bpy = 2,2'-联吡啶，phen = 1,10-菲咯啉），（7-硝基-dppz = 7-硝基二吡啶并[3,2-a:2'-3'-c]吩嗪，7-CH(3)-dppz = 7-甲基二吡啶并[3,2-a:2'-3'-c]吩嗪），并通过红外光谱、紫外光谱、元素分析、(1)H核磁共振、(13)C-核磁共振和质谱对其进行了表征。通过光谱和粘度测量研究了这四种配合物与DNA的结合特性。结果表明，所有四种配合物均通过插入模式与DNA结合。在365 nm光照下，发现所有四种配合物均能促进质粒pBR 322 DNA的光裂解。对所选配合物的毒理学效应在具有工业重要性的酵母（真核微生物）上进行了研究。
Zotero 插件