Carlsen N L, Mortensen B T, Andersson P K, Larsen J K, Nielsen O H, Tommerup N
Department of Internal Medicine and Haematology, Finsen Institute, Copenhagen, Denmark.
Anticancer Res. 1991 Jan-Feb;11(1):353-8.
The association of ability to grow in vitro, non-random chromosome abnormalities, DNA ploidy and oncogene amplification with prognosis may lead to a better understanding of the biology of neuroblastomas. In a pilot study fresh tumour tissue was obtained from 4 consecutive patients at the Department of Paediatric Surgery, Rigshospitalet, Denmark. Chromosome abnormalities were detected in 3 out of 3 successfully karyotyped tumours, and one or more aneuploid stem lines were detected in 4 out of 4 tumours using flow cytometry. The N-myc oncogene was amplified in 1 out of 3 tumours tested. Continuous cell lines could be established from all 3 advanced stage tumours. This pilot study has shown that fresh tumour tissue can be obtained and successfully studied for various fundamental aspects of the biology of neuroblastomas.
体外生长能力、非随机染色体异常、DNA倍体和癌基因扩增与预后的关联可能有助于更好地理解神经母细胞瘤的生物学特性。在一项试点研究中，从丹麦里格霍斯皮塔尔儿科外科的4例连续患者中获取了新鲜肿瘤组织。在3例成功进行核型分析的肿瘤中，检测到染色体异常，并且使用流式细胞术在4例肿瘤中的4例中检测到一个或多个非整倍体干细胞系。在3例检测的肿瘤中有1例检测到N-myc癌基因扩增。可以从所有3例晚期肿瘤中建立连续细胞系。这项试点研究表明，可以获取新鲜肿瘤组织并成功地对神经母细胞瘤生物学的各个基本方面进行研究。
