Neuroscience Research Unit, Puerta de Hierro Hospital, 28222 Majadahonda, Madrid, Spain.
Histol Histopathol. 2010 Apr;25(4):453-61. doi: 10.14670/HH-25.453.
When a severe neurological lesion occurs as a consequence of intracerebral bleeding, no effective treatment for improving the outcome is currently available. In the present study, intracerebral hemorrhage (ICH) was induced by stereotactic injection of 0.5 U of collagenase type IV in the striatum of adult Wistar rats, and three days later, intralesional administration of 2 x 10(6) allogeneic bone marrow stromal cells (BMSC) in saline (n:10), or saline only (n:10), was performed. In the following 30 days, functional outcome was evaluated in each animal by rotarod and the modified neurological severity score (mNSS) test. Progressive and functional improvement was observed in BMSC-transplanted rats compared with controls, together with morphological images suggesting that intracerebral administration of BMSC increases endogenous neurogenesis at the level of subventricular zone (SVZ). These findings suggest that local administration of allogeneic BMSC could be useful to reduce the neurological deficits caused by intracerebral hemorrhage.
当由于脑出血而发生严重的神经损伤时，目前尚无有效的治疗方法可以改善预后。在本研究中，通过立体定向注射 0.5U 胶原酶 IV 在成年 Wistar 大鼠纹状体中诱导脑出血，3 天后，在病灶内给予 2x10(6)个同种异体骨髓基质细胞（BMSC）生理盐水（n=10）或仅生理盐水（n=10）。在接下来的 30 天内，通过旋转棒和改良神经严重程度评分（mNSS）测试对每个动物的功能结果进行评估。与对照组相比，BMSC 移植大鼠表现出进行性和功能性改善，形态学图像表明，脑内给予 BMSC 可增加侧脑室下区（SVZ）水平的内源性神经发生。这些发现表明，局部给予同种异体 BMSC 可能有助于减少脑出血引起的神经功能缺损。
