Department of Neurobiology & Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA.
Cytokine. 2010 Jun;50(3):317-21. doi: 10.1016/j.cyto.2010.01.004. Epub 2010 Feb 24.
Mesenchymal stem cells (MSC) derived from bone marrow are ideal transplants for a variety of CNS disorders and appear to support recovery after injury by secreting therapeutic factors. There is considerable variability in the secretion profile of MSC derived from different donors and it is known that MSC secretion changes in response to inflammatory stimuli, but no comprehensive analysis has been performed to address these issues. Here we show that MSC from seven donors secrete chemokines and cytokines in variable ranges, with some factors showing high variability. Treatment of cultured MSC with pro-inflammatory cytokines or tissue extracts from injured spinal cord resulted in up-regulation of selected cytokines, whereas treatment with an anti-inflammatory cytokine had little effect, indicating that the secretion profile is tightly regulated by environmental challenges. Patterns of up-regulated cytokines were similar in MSC from different donors suggesting a comparable response to inflammatory stimuli.
骨髓间充质干细胞（MSC）是治疗多种中枢神经系统疾病的理想移植物，通过分泌治疗因子来支持损伤后的恢复。不同供体来源的 MSC 分泌谱存在很大差异，并且已知 MSC 分泌会响应炎症刺激而改变，但尚未进行全面分析以解决这些问题。在这里，我们显示来自七个供体的 MSC 以不同范围分泌趋化因子和细胞因子，其中一些因子表现出高度的可变性。用促炎细胞因子或损伤脊髓的组织提取物处理培养的 MSC 会导致选定细胞因子的上调，而用抗炎细胞因子处理则几乎没有影响，表明分泌谱受环境挑战的严格调节。不同供体来源的 MSC 中上调细胞因子的模式相似，表明对炎症刺激有类似的反应。
