Department of Biotechnology and Adv Lab for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology, Kharagpur 721302, India.
Biochem Biophys Res Commun. 2010 Mar 26;394(1):178-83. doi: 10.1016/j.bbrc.2010.02.142. Epub 2010 Feb 24.
The functional role of rice (Oryza sativa) germin-like protein1 (OsGLP1) was elucidated through development of transgenic plants involving endogenous gene silencing in rice and heterologous gene expression in tobacco. Usually, the single copy OsGLP1 gene in rice plant was found to be expressed predominantly in green vegetative tissues. The transgenic rice lines showed significant reduction in endogenous OsGLP1 expression due to 26nt siRNA-mediated gene silencing, displayed semi-dwarfism and were affected seriously by fungal diseases, compared to the untransformed plant. Structural homology modeling predicted a superoxide dismutase (SOD) domain in OsGLP1 protein which upon over-expression in transgenic tobacco plant clearly documented SOD activity. Our observations on the maintenance of cell dimension, cell wall-associated localization particularly in the sub-epidermal tissues and the SOD activity of OsGLP1 could explain its functional role in regulation of plant height and disease resistance in rice plant.
通过涉及水稻内源基因沉默的转基因植物和烟草异源基因表达的方法,阐明了水稻(Oryza sativa)萌发相关蛋白 1(OsGLP1)的功能作用。通常,水稻植株中的单拷贝 OsGLP1 基因主要在绿色营养组织中表达。与未转化的植株相比,由于 26nt siRNA 介导的基因沉默,转基因水稻系表现出内源性 OsGLP1 表达的显著减少,表现出半矮化,并且受到真菌病的严重影响。结构同源建模预测 OsGLP1 蛋白中存在超氧化物歧化酶(SOD)结构域,在转基因烟草植物中的过表达清楚地记录了 SOD 活性。我们观察到 OsGLP1 维持细胞维度、细胞壁相关定位,特别是在表皮组织下,以及 SOD 活性,这可以解释其在调节水稻株高和抗病性方面的功能作用。