Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 Beier Road, Heping District, Shenyang, Liaoning 110001, People's Republic of China.
Ecotoxicol Environ Saf. 2010 Sep;73(6):1323-6. doi: 10.1016/j.ecoenv.2010.02.012. Epub 2010 Feb 25.
The aim of this study was to explore distribution of speciated arsenicals in mice exposed to arsenite at early developmental stages. Levels of speciated arsenicals in both liver and brain of mice were analyzed by hydride generation of volatile arsines, and determined by atomic absorption spectrophotometry (HG-AAS). In the liver, levels of inorganic arsenic (iAs) increased on postnatal day (PND) 15, and monomethylarsonic acid (MMA) increased on PND 21, however, levels of dimethylarsinic acid (DMA) in newborn mice were significantly higher than those on PND 10 and 15. In the brain, levels of iAs on PND 21 were the highest; iAs levels on PND 15 were also significantly higher than those on PND 35. Our results suggested transplacental transfer of arsenicals from pregnant mice into their fetus was relatively efficient, lactational transfer from mother mice into their offspring was inefficient, and transfer of iAs from blood into brain at early developmental stages was efficient.
本研究旨在探讨亚砷酸盐在早期发育阶段暴露于亚砷酸盐的小鼠体内的形态分布。采用氢化物发生挥发性砷的方法对小鼠肝、脑中的形态砷进行分析，并采用原子吸收分光光度法（HG-AAS）进行测定。在肝脏中，无机砷（iAs）的水平在出生后第 15 天增加，单甲基砷酸（MMA）的水平在第 21 天增加，然而，新生小鼠中二甲基砷酸（DMA）的水平明显高于第 10 天和第 15 天。在大脑中，第 21 天的 iAs 水平最高；第 15 天的 iAs 水平也明显高于第 35 天。我们的结果表明，母体向胎儿的砷形态转移相对有效，母鼠向其后代的乳转移效率较低，早期发育阶段 iAs 从血液向大脑的转移效率较高。
