从水溶液中电沉积透明质酸和透明质酸-牛血清白蛋白膜。

Electrodeposition of hyaluronic acid and hyaluronic acid-bovine serum albumin films from aqueous solutions.

机构信息

Department of Materials Science and Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4L7.

出版信息

Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):279-85. doi: 10.1016/j.colsurfb.2010.02.002. Epub 2010 Feb 12.

DOI:10.1016/j.colsurfb.2010.02.002

PMID:20188528

Abstract

Hyaluronic acid (HYH) films were prepared from aqueous sodium hyaluronate (HYNa) solutions by anodic electrodeposition. The film thickness was varied in the range of 0-20 microm by the variation of the deposition time and HYNa concentration. The deposition rate was low at HYNa concentration below 1 g L(-1) and increased significantly in the range of 3-5 g L(-1). The addition of bovine serum albumin (BSA) to the HYNa solutions resulted in increased deposition yield, which was attributed to the formation of composite HYH-BSA films. The thickness of the HYH-BSA films deposited by anodic electrodeposition was varied in the range of 0-80 microm. The HYH and composite HYH-BSA films were studied by scanning electron microscopy, thermogravimetric analysis, differential thermal analysis, Fourier transform infrared spectroscopy and circular dichroism spectroscopy. The deposition mechanism and kinetics of deposition are discussed.

摘要

透明质酸（HYH）薄膜通过阳极电沉积法从含透明质酸钠（HYNa）的水溶液中制备。通过改变沉积时间和 HYNa 浓度，可以将薄膜厚度控制在 0-20 微米的范围内。当 HYNa 浓度低于 1g/L 时，沉积速率较低，而在 3-5g/L 的范围内则显著增加。将牛血清白蛋白（BSA）添加到 HYNa 溶液中会导致沉积产率增加，这归因于复合 HYH-BSA 薄膜的形成。通过阳极电沉积法沉积的 HYH-BSA 薄膜的厚度可以在 0-80 微米的范围内变化。通过扫描电子显微镜、热重分析、差示热分析、傅里叶变换红外光谱和圆二色光谱研究了 HYH 和复合 HYH-BSA 薄膜。讨论了沉积的沉积机制和动力学。