Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av Trabalhador Sãocarlense 400, 13566-590 São Carlos, SP, Brazil.
Bioresour Technol. 2010 Jul;101(14):5112-22. doi: 10.1016/j.biortech.2010.01.141. Epub 2010 Mar 1.
Four anaerobic fluidized bed reactors filled with activated carbon (R1), expanded clay (R2), glass beads (R3) and sand (R4) were tested for anaerobic degradation of LAS. All reactors were inoculated with sludge from a UASB reactor treating swine wastewater and were fed with a synthetic substrate supplemented with approximately 20 mg l(-1) of LAS, on average. To 560 mg l(-1) COD influent, the maximum COD and LAS removal efficiencies were mean values of 97+/-2% and 99+/-2%, respectively, to all reactors demonstrating the potential applicability of this reactor configuration for treating LAS. The reactors were kept at 30 degrees C and operated with a hydraulic retention time (HRT) of 18h. The use of glass beads and sand appear attractive because they favor the development of biofilms capable of supporting LAS degradation. Subsequent 16S rRNA gene sequencing and phylogenetic analysis of samples from reactors R3 and R4 revealed that these reactors gave rise to broad microbial diversity, with microorganisms belonging to the phyla Bacteroidetes, Firmicutes, Actinobacteria and Proteobacteria, indicating the role of microbial consortia in degrading the surfactant LAS.
四个填充有活性炭（R1）、膨胀粘土（R2）、玻璃珠（R3）和沙子（R4）的厌氧流化床反应器被用于测试 LAS 的厌氧降解。所有反应器均接种了来自处理猪废水的 UASB 反应器的污泥，并以平均约 20mg l(-1) 的 LAS 补充的合成基质进行进料。对于 560mg l(-1) COD 进水，最大 COD 和 LAS 去除效率分别为 97+/-2%和 99+/-2%，所有反应器均表明这种反应器配置用于处理 LAS 的潜在适用性。反应器保持在 30 摄氏度，并以 18 小时的水力停留时间（HRT）运行。使用玻璃珠和沙子似乎很有吸引力，因为它们有利于能够支持 LAS 降解的生物膜的发展。来自 R3 和 R4 反应器的样品的后续 16S rRNA 基因测序和系统发育分析表明，这些反应器产生了广泛的微生物多样性，微生物属于拟杆菌门、厚壁菌门、放线菌门和变形菌门，表明微生物群落在降解表面活性剂 LAS 中的作用。
