Filipiuc Silvia, Nicolae Odette, Luncă Cătălina, Iancu Luminiţa Smaranda
Laboratorul de Microbiologie, Direcţia de Sănătate Publică Suceava.
Rev Med Chir Soc Med Nat Iasi. 2009 Oct-Dec;113(4):1238-42.
Neisseria gonorrhoeae infection is one of the most frequent infections transmitted on sexual intercourse. In the last decades, a significant numbers of gonococcus strains were resistant to penicillin, tetracycline, quinolones and, in the last period, resistant to 3rd generation cephalosporines too. The purpose of this study was to monitor the trend of N. gonorrhoeae isolates from 2004-2008, in Suceava County, North-East region of Romania.
Antimicrobial susceptibility of N. gonorrhoeae isolates from clinical cases in 48 males and 12 females to penicillin, tetracycline, amoxicillin, augmentin, clarithromycine, ciprofloxacin, and ceftriaxone, was determine by disc diffusion method, using WHO reference strains and NCCLS criteria of interpretation. The isolation and identification of N. gonorrhoeae strains was carried out using classical microbiological algorithm, and miniAPI galleries.
A high proportion of strains were resistant to tetracycline and penicillin (70 and 80% respectively); 95% of strains were sensitive to ceftriaxone, one strains was resistant and 2 of them (3.33%), intermediate resistant. For all other tested antibiotics the level of resistant strains varied from 55 to 65%.
The present study is the first one regarding the level of antibiotics resistance of N. gonorrhoeae strains in Suceava County. Penicillin, tetracycline, ciprofloxacin, clarithromycine, amoxicillin, and augmentin can not be indicated as treatment of N. gonorrhoeae infection because of high level of resistance. An active and comprehensive studies for monitoring and surveillance of antimicrobial resistance of N. gonorrhoeae needs to be established in all regions of the country, as support of new therapeutical treatment scheme.
淋病奈瑟菌感染是通过性交传播的最常见感染之一。在过去几十年中，大量淋病菌株对青霉素、四环素、喹诺酮类药物产生耐药性，并且在最近一段时间，也对第三代头孢菌素产生了耐药性。本研究的目的是监测罗马尼亚东北部苏恰瓦县2004年至2008年淋病奈瑟菌分离株的趋势。
采用纸片扩散法，使用世界卫生组织参考菌株和美国国家临床实验室标准委员会（NCCLS）的解释标准，测定48例男性和12例女性临床病例中淋病奈瑟菌分离株对青霉素、四环素、阿莫西林、奥格门汀、克拉霉素、环丙沙星和头孢曲松的抗菌敏感性。淋病奈瑟菌菌株的分离和鉴定采用经典微生物学算法和微量API鉴定板进行。
很大比例的菌株对四环素和青霉素耐药（分别为70%和80%）；95%的菌株对头孢曲松敏感，1株耐药，2株（3.33%）中介耐药。对于所有其他测试抗生素，耐药菌株水平在55%至65%之间。
本研究是关于苏恰瓦县淋病奈瑟菌菌株抗生素耐药水平的首次研究。由于耐药水平高，青霉素、四环素、环丙沙星、克拉霉素、阿莫西林和奥格门汀不能用于治疗淋病奈瑟菌感染。作为新治疗方案的支持，需要在该国所有地区开展积极全面的淋病奈瑟菌抗菌耐药性监测研究。
