DNA甲基转移酶1基因单核苷酸多态性对伊朗胃癌患者无影响：一项病例对照研究。
Lack of effects of single nucleotide polymorphisms of the DNA methyltransferase 1 gene on gastric cancer in Iranian patients: a case control study.
作者信息
Khatami Fatemeh, Noorinayer Babak, Ghiasi Somayeh, Mohebi Reza, Hashemi Mohhamad, Zali Mohammad Reza
机构信息
Research Center for Gastrointestinal and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Asian Pac J Cancer Prev. 2009;10(6):1177-82.
BACKGROUND
Gastric cancer is one of the most common malignant tumors in Iran. Hypomethylation and/or hypermethylation of DNA has been described in gastric cancer and is presumed to be an early event in carcinogeneisis.
OBJECTIVE
We therefore hypothesized that single nucleotide polymorphisms of the DNMT1 gene may be associated with the genetic susceptibility to gastric cancer.
METHODS
Totals of 200 patients and 200 controls, both of Iranian origin, were studied. Three polymorphisms were genotyped by PCR-RFLP and allele frequencies and genotypes were compared between the cases and controls. Odds ratios were calculated and the interactions between the polymorphisms, age and sex were examined.
RESULTS
There were no significant associations between the DNMT1 polymorphisms and gastric cancer.
CONCLUSION
We could not show any association between DNMT1 polymorphisms and gastric cancer. Larger sets of polymorphisms and sample sizes are required for future testing of possible associations.
背景
胃癌是伊朗最常见的恶性肿瘤之一。DNA的低甲基化和/或高甲基化已在胃癌中被描述，并且被认为是致癌过程中的早期事件。
目的
因此，我们假设DNA甲基转移酶1（DNMT1）基因的单核苷酸多态性可能与胃癌的遗传易感性相关。
方法
研究了总共200例患者和200例对照，二者均为伊朗裔。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对三种多态性进行基因分型，并比较病例组和对照组之间的等位基因频率和基因型。计算优势比，并检查多态性、年龄和性别之间的相互作用。
结果
DNMT1基因多态性与胃癌之间无显著关联。
结论
我们未能显示DNMT1基因多态性与胃癌之间存在任何关联。未来需要更大规模的多态性和样本量来测试可能的关联。
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