Wang Zhen, Rong Xin-zhou, Zhang Tao, Yang Rong-hua
Department of Burns, Guangzhou First Municipal People's Hospital, Guangzhou Medical College, Guangzhou 510180, China.
Zhonghua Shao Shang Za Zhi. 2009 Dec;25(6):441-3.
To observe the change in albumin concentration in the subeschar tissue fluid of rabbits in early stage after burn, and to analyze its regular pattern.
Thirty-four adult male New Zealand rabbits were divided into control group and experiment group according to the random number table, with 17 rabbits in each group. Rabbits in experiment group were subjected to 8% TBSA full-thickness scald on the back and were injected with human serum albumin in subeschar tissue serving as tracing albumin. 1.5 mL blood sample was collected at post scald hour (PSH) 2, 4, 8, 16, 24, 48, 72 respectively. Rabbits in control group were dealt with the above-mentioned procedures except for scald. The concentration of tracing albumin was measured with the enzyme-linked immunosorbent assay kit. The concentration of the serum albumin of rabbits were determined with biochemical analyzer. Pharmacokinetics parameters of tracing albumin were calculated with fitting model of 3P97 practical pharmacokinetics calculating program.
(1) Concentration of tracing albumin of rabbits in experiment group was respectively higher than that in control group (P < 0.01) at each time point, and it peaked at PSH 8 [(421 +/- 10) microg/L]. (2) The concentration of serum albumin of rabbits in experiment group decreased in the beginning and increased later, while no significant change was observed in control group. (3) The distribution phase half-life of tracing albumin of rabbits in experiment group (4.0271 h) was about 1/3 of that of the control group (12.0907 h); while the area under the curve in the experiment group (22 336.38 microg.h.mL(-1)) was about 4 times of that in the control group (5827.77 microg.h.mL(-1)).
The albumin in the subcutaneous tissue could be absorbed into blood circulation in normal conditions. The resorption occurs earlier and faster and more when obvious inflammation occurs (such as deep burn). Exudation and resorption of albumin co-exist in the early stage after burn.
观察兔烧伤早期焦痂下组织液中白蛋白浓度的变化，并分析其规律。
34只成年雄性新西兰兔按随机数字表法分为对照组和实验组，每组17只。实验组兔背部行8%TBSA全层烫伤，并于焦痂下组织注射人血清白蛋白作为示踪白蛋白。分别于烫伤后2、4、8、16、24、48、72小时采集1.5 mL血样。对照组兔除不烫伤外，行上述相同操作。用酶联免疫吸附测定试剂盒测定示踪白蛋白浓度。用生化分析仪测定兔血清白蛋白浓度。用3P97实用药代动力学计算程序拟合模型计算示踪白蛋白的药代动力学参数。
（1）实验组兔各时间点示踪白蛋白浓度均高于对照组（P<0.01），于烫伤后8小时达峰值[（421±10）μg/L]。（2）实验组兔血清白蛋白浓度初期降低，随后升高，而对照组未见明显变化。（3）实验组兔示踪白蛋白的分布相半衰期（4.0271小时）约为对照组（12.0907小时）的1/3；而实验组曲线下面积（22 336.38μg·h·mL-1）约为对照组（5827.77μg·h·mL-1）的4倍。
正常情况下皮下组织中的白蛋白可吸收入血液循环。在明显炎症（如深度烧伤）时，吸收发生得更早、更快且更多。烧伤早期白蛋白渗出与吸收并存。
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