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PsbK 亚基是嗜热蓝细菌 Thermosynechococcus elongatus BP-1 中 PSII 复合物中其他小亚基稳定组装和稳定所必需的。

The PsbK subunit is required for the stable assembly and stability of other small subunits in the PSII complex in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1.

机构信息

Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Yamazaki 2641, Noda, Chiba, 278-8510 Japan.

出版信息

Plant Cell Physiol. 2010 Apr;51(4):554-60. doi: 10.1093/pcp/pcq020. Epub 2010 Mar 1.

Abstract

PsbK is a small membrane protein of the PSII core complex and is highly conserved from cyanobacteria to plants. Here, we studied its role in the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by focusing on a psbK disruptant with hexahistidine-tagged CP47. The psbK disruptant showed photoautotrophic growth comparable with that of the wild type under a wide range of light conditions. The mutant PSII complex retained the oxygen-evolving activity with a unique modification of the acceptor Q(B) site. N-terminal sequencing revealed that Ycf12 and PsbZ proteins were lost in the PSII complex prepared from the mutant. Immunoblotting detected reduced accumulation of PsbZ in the mutant thylakoid. These results suggest that PsbK is required not only for association of PsbZ and Ycf12 with the isolated PSII complex but also for the stabilization of PsbZ in the thylakoid membrane.

摘要

PsbK 是 PSII 核心复合物的一种小膜蛋白,在从蓝藻到植物的范围内高度保守。在这里,我们通过专注于带有六组氨酸标记 CP47 的 psbK 缺失突变体来研究其在嗜热蓝藻 Thermosynechococcus elongatus BP-1 中的作用。psbK 缺失突变体在广泛的光照条件下显示出与野生型相当的光自养生长。突变 PSII 复合物保留了产氧活性,具有独特的受体 Q(B) 位点修饰。N 端测序表明,在突变体 PSII 复合物中,Ycf12 和 PsbZ 蛋白丢失。免疫印迹检测到突变体类囊体中 PsbZ 的积累减少。这些结果表明,PsbK 不仅需要 PsbZ 和 Ycf12 与分离的 PSII 复合物的结合,而且需要 PsbZ 在类囊体膜中的稳定。

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