Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Opt Lett. 2010 Mar 1;35(5):781-3. doi: 10.1364/ol.35.000781.
A wavelength-coded multifocal microscope incorporating multiplexed and wavelength-coded holographic gratings to generate wavelength-selective multifocal planes is presented. The focal planes are longitudinally spaced on the object plane, and each focal plane is probed by a designated wavelength. The recording of the multiplexed gratings takes place at a single wavelength by utilizing the Bragg degeneracy property; thus the maximum sensitive wavelength of blue 488 nm is used for recording, but the device is operated at a broad wavelength band of interest, all the way to red 633 nm. We present the design, implementation, and experimental image data demonstrating this microscope's ability to obtain biological tissue structures simultaneously at different focal planes using broadband illumination by LEDs.
提出了一种波长编码的多焦点显微镜，它结合了复用和波长编码全息光栅，以产生波长选择性的多焦点平面。焦点平面在物平面上纵向间隔开，每个焦点平面由指定的波长探测。通过利用布拉格简并特性，在单个波长下记录复用光栅，因此使用最大敏感波长 488nm 的蓝光进行记录，但该设备在感兴趣的宽波长带中运行，一直到 633nm 的红光。我们介绍了这种显微镜的设计、实现和实验图像数据，展示了它使用 LED 宽带照明在不同焦点平面上同时获取生物组织结构的能力。
