Department Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1, 20126 Milan, Italy.
Appl Microbiol Biotechnol. 2010 Jun;87(1):297-308. doi: 10.1007/s00253-010-2497-3. Epub 2010 Mar 2.
Rhodococcus opacus R7 is a naphthalene-degrading microorganism which is also able to grow on o-xylene. This work describes the isolation and analysis of two new genomic regions in which genes involved in naphthalene (nar gene cluster) and salicylate (gen gene cluster) degradation are located. In the nar gene cluster we found: two genes encoding the large (narAa) and the small (narAb) components of the naphthalene dioxygenase, three genes (rub1, rub2, rub1bis) encoding three rubredoxins, an orf (orf7) associated to the complex encoding a protein of unknown function, two regulatory genes (narR1, narR2), a gene (narB) encoding the naphthalene dihydrodiol dehydrogenase and six orfs (orf1, orf2, orf3, orf4, orf5, orf6) encoding proteins of unknown function. In the gen gene cluster, we found the following genes: two genes encoding the salicylate CoA ligase and the salicylate CoA synthetase (genA and genB), respectively, a gene (genC) encoding a salicylate hydroxylase, a gene (genH) encoding a gentisate 1,2-dioxygenase, a gene (genI) encoding a 3-maleylpyruvate isomerase, and a gene (genL) encoding a protein of unknown function. The transcription of some genes of R. opacus R7 strain grown on different substrates was also investigated to evaluate the expression of the two gene clusters after cDNA preparations.
红球菌属 R7 是一种能够降解萘的微生物，也能够以邻二甲苯为生长基质。本研究描述了两个新的基因组区域的分离和分析，其中包含萘（nar 基因簇）和水杨酸（gen 基因簇）降解相关的基因。在 nar 基因簇中，我们发现了两个编码萘二氧合酶大亚基（narAa）和小亚基（narAb）的基因，三个编码三种 rubredoxins 的基因（rub1、rub2、rub1bis），一个与编码未知功能蛋白复合物相关的开放阅读框（orf7），两个调控基因（narR1、narR2），一个编码萘二氢二醇脱氢酶的基因（narB）和六个编码未知功能蛋白的开放阅读框（orf1、orf2、orf3、orf4、orf5、orf6）。在 gen 基因簇中，我们发现了以下基因：两个编码水杨酸 CoA 连接酶和水杨酸 CoA 合成酶的基因（genA 和 genB），一个编码水杨酸羟化酶的基因（genC），一个编码 gentisate 1,2-双加氧酶的基因（genH），一个编码 3-顺乌头酸丙酮酸异构酶的基因（genI），和一个编码未知功能蛋白的基因（genL）。我们还研究了 R. opacus R7 菌株在不同基质上生长时的某些基因转录情况，以评估在 cDNA 制备后两个基因簇的表达情况。
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