[Mutations in the Sup35 gene impairs degradation of mRNA containing premature stop codons].

Eukaryotic cells possess special mechanism of the degradation of mRNAs containing premature termination codons (PTCs)--nonsense-mediated mRNA decay (NMD) pathway. In yeast Saccharomyces cerevisiae the activity of this pathway depends on the recognition of the PTC by the translational machinery and interaction of translation termination factors eRF1 (Sup45) and eRF3 (Sup35) with Upfl, Upf2 and Upf3 proteins. Previously we have shown that decreasing of eRF1 amount causes an impairment of NMD. Here we show that sup35 nonsense and missense mutations lead to accumulation of PTC-containing transcripts such as his7-1 mRNA and CYH2 pre-mRNA. Thus sup35 mutations do not only decrease translation fidelity but also influence mRNA stability. Remarkably, deletion of either UPF1 or UPF2 increased viability of sup35 mutants, while UPF3 deletion leads to decreased viability of sup35 mutants.